Chchd10基因在睾丸发育过程中的表达谱。

IF 1.1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2025-05-12 DOI:10.1016/j.gep.2025.119395

Xiao Jiang , Shengqi Sun , Fei Han , Lei Sun , Jing Gu , Jia Cao , Jinyi Liu

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引用次数: 0

摘要

Chchd10蛋白对维持线粒体动力学、生理和功能至关重要，据报道在心肌细胞和骨骼肌中含量最多。然而，Chchd10在性腺发育中的表达模式尚不清楚。在这里，我们利用公开的数据集描述了Chchd10基因在小鼠胚胎性腺发育和出生后睾丸发育中的表达模式，以及Chchd10基因在人类青春期睾丸和青年成年睾丸中的表达模式。此外，我们利用RT-qPCR和免疫荧光技术研究了Chchd10在小鼠睾丸中的表达和分布，并分析了Chchd10在睾丸中的可能作用和机制。我们注意到Chchd10在胚胎睾丸中比在卵巢中表达丰富，并在小鼠胚胎和出生后睾丸发育过程中动态表达。此外，Chchd10在胚胎和出生后小鼠睾丸支持细胞群体中含量都很高，并且主要位于小鼠支持细胞的线粒体中。此外，CHCHD10不仅在人青春期睾丸和成人睾丸的支持细胞中富集，而且在tMΦ中也有高表达。CHCHD10可能通过调节支持细胞的多种生物学过程参与睾丸发育。综上所述，我们的数据表明Chchd10似乎在睾丸发育过程中很重要，特别是在支持细胞的功能调节中。本研究首次揭示了Chchd10基因在睾丸发育过程中的表达谱，将为进一步研究Chchd10的功能和分子机制提供新的思路。

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Expression profile of Chchd10 gene during testicular development

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Expression profile of Chchd10 gene during testicular development

Chchd10 protein is crucial for sustaining mitochondrial dynamics, physiology and functions, and has been reported to be most abundantly in myocardial cells and skeletal muscle. However, nothing is known for the expression pattern of Chchd10 in gonadal development. Here, we characterized the expression patterns of Chchd10 gene during embryonic gonad development and postnatal testis development in mice, as well as the expression pattern of Chchd10 gene in human puberty testis and young adult testis using publicly available datasets. Besides, we investigated the expression and distribution of Chchd10 in mice testis by RT-qPCR and immunofluorescence and analyzed the possible role and mechanism of Chchd10 in the testis. We noticed that Chchd10 showed abundant expression in embryonic testis compared to ovaries and dynamically expressed during embryonic and postnatal testis development in mice. In addition, Chchd10 was highly abundant within testicular Sertoli cells populations both in embryonic and postnatal mice and mainly located in the mitochondria of Sertoli cells in mice. Furthermore, CHCHD10 was not only enriched in Sertoli cells, but also highly expressed in tMΦ of human puberty testis and adult testis. CHCHD10 may participate in testicular development by regulating multiple biological processes of Sertoli cells. Taken together, our data indicated that Chchd10 appears to be important during testicular development, particularly in the functional modulation of Sertoli cells. Our study revealed the expression profile of Chchd10 gene during testicular development for the first time and will provide new ideas for further studying the function and molecular mechanism of Chchd10.

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation