秋葵黑叶霉病原菌abelmoschi的全基因组测序与注释。

IF 4 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Shagun Sinha, Sudhir Navathe, Anjali, Shubham Vishwakarma, Priyanka Prajapati, Ramesh Chand, Ravindra Nath Kharwar
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引用次数: 0

摘要

阿贝尔莫氏假cercospora会在秋葵的叶子上引起黑霉。该病流行于雨季过后,此时湿度高、温度高。在有利的环境中观察到严重的落叶,导致生产力的重大损失。基于该病原菌在农业上的重要性,选择abelmoschi分离菌Cer 86 - 18 (MCC:9491)进行基因组测序。abelmoschi p.a belmoschi基因组全长31.90 Mb,总GC含量为54.26%。使用BUSCO (Benchmarking Universal Single-Copy Orthologs)进行定量基因组评估,鉴定出1,664个(97.53%)完整的BUSCO,反映了具有最小重复和强同源独特性的保守基因的高度代表性。基因预测分析鉴定出11325个蛋白质编码基因,其中3857个使用KEGG数据库进行了注释。结果表明,有410个基因编码碳水化合物活性酶,369个基因编码肽酶。还鉴定了18个参与次生代谢物生物合成的基因簇。总共有143个蛋白被预测为利用硅管道的效应器。这是关于阿贝尔莫氏疟原虫基因组组织的第一份报告。本研究旨在通过提高我们对阿贝尔莫氏疟原虫基因组组织和基因注释的理解来解决这一空白,从而为功能基因组学研究铺平道路,例如鉴定毒力基因以帮助抗性育种。此外,该基因组可能是Pseudocercospora属可用基因组资源的又一补充,并可以为宿主-病原体相互作用和进化关系提供有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Whole genome sequencing and annotation of Pseudocercospora abelmoschi, a causal agent of black leaf mould of okra.

Pseudocercospora abelmoschi causes black mould on the leaves of okra. The disease is prevalent post-rainy season when high moisture and warm temperatures prevail. Severe defoliation is observed during favourable environments, leading to a significant loss in productivity. Based on the importance of the pathogen agriculturally, the P. abelmoschi isolate Cer 86 - 18 (MCC:9491) was selected for genome sequencing. The genome assembly of P. abelmoschi resulted in a genome of 31.90 Mb with an overall GC content of 54.26%. Quantitative genome assessment using BUSCO (Benchmarking Universal Single-Copy Orthologs) identified 1,664 (97.53%) complete BUSCOs, reflecting a high representation of conserved genes with minimal duplication and strong orthologous uniqueness. Gene prediction analysis identified 11,325 protein-coding genes, of which 3,857 were annotated using the KEGG database. As per analyses, 410 genes were predicted to encode carbohydrate-active enzymes, whereas 369 genes were predicted to encode peptidases. Eighteen gene clusters involved in secondary metabolite biosynthesis were also identified. A total of 143 proteins were predicted to be effectors using the in-silico pipeline. This is the first report on the genome organisation of P. abelmoschi. This study was designed to address this gap by enhancing our understanding of the genome organisation of P. abelmoschi and gene annotation, thereby paving the way for functional genomics studies, such as identifying virulence genes to aid in resistance breeding. Also, this genome could be another addition to the available genomic resources of the genus Pseudocercospora and can provide valuable insights into host-pathogen interactions and evolutionary relationships.

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来源期刊
World journal of microbiology & biotechnology
World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物
CiteScore
6.30
自引率
2.40%
发文量
257
审稿时长
2.5 months
期刊介绍: World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.
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