Impact of Mulberry Leaves on CCL4-Induced Acute Liver Disease in Rats.

<b>Background and Objective:</b> The liver is a crucial organ in the human body that contributes to metabolic processes, immune function and nutrient regulation. The objective of this study is to evaluate the effects of mulberry leaves (<i>Morus nigra</i> L.) on rats with acute liver injury induced by CCl₄. <b>Materials and Methods:</b> Twenty-four male albino Sprague Dawley rats (~195±10 g) were divided into control and CCl₄-induced liver injury groups. The treated group received a monthly basal diet of 2.5 and 5% mulberry leaves. Liver histopathology, body weight, organ-to-body weight ratios and feed intake were assessed. Lipid profiles and serum markers (glucose, urea, AST and ALT) were analyzed using SPSS (Mean±SD) with a significance level of p<0.05. <b>Results:</b> Total serum cholesterol and blood triglyceride levels were significantly lower (p<0.05) in all mulberry-fed groups, with the most notable reduction in the 5% group. The 5% mulberry leaf group also observed the best lipoprotein profile. The 5% mulberry leaf diet improved AST, ALT and serum glucose levels in rats with acute liver disease. <b>Conclusion:</b> Based on the results shown here, it appears that taking 5% ML daily can help improve liver functioning and shield rats from acute damage caused by CCL₄.