食物中氧化石墨烯对几代家蟋蟀消化酶活性的影响。

IF 3.6 3区 医学 Q3 NANOSCIENCE & NANOTECHNOLOGY
Agnieszka Babczyńska, Monika Tarnawska, Klaudia Czaja, Barbara Flasz, Amrendra K Ajay, Łukasz Napora-Rutkowski, Katarzyna Rozpędek, Ewa Świerczek, Andrzej Kędziorski, Maria Augustyniak
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引用次数: 0

摘要

增加纳米粒子或纳米材料的使用可能导致它们释放到环境中。这些结构物的毒性,被归类为新出现的污染物,还没有得到充分的了解。然而,就像其他环境压力源一样,暴露于它们的影响应该在多代人的尺度上进行分析,以预测暴露于它们的人群的后果。因此,本项目旨在评估氧化石墨烯(GO)纳米材料对作为模式物种的家蟋蟀(Acheta domesticus)消化道酶活性的影响,这取决于氧化石墨烯浓度(食物干重0.2或0.02µg·g-1)、先前的长寿选择以及自接触开始以来发生的世代数(1-5)。最后一代和第六代是将氧化石墨烯从饮食中剔除的昆虫(恢复一代)。酶活性检测采用API酶酶试剂盒改进的分光光度法读数。测试显示氧化石墨烯干扰了一些消化酶。此外,氧化石墨烯的影响取决于种群先前的长寿选择。减轻衰老后果的机制的影响支持对氧化石墨烯中毒的可能耐受。这在野生和长寿昆虫对氧化石墨烯的多代反应的不同模式中得到了证明。此外,几代人的接触也揭示了“第三代”效应。最后,氧化石墨烯消除的影响取决于用于测试的纳米材料的浓度。此外,在激效的背景下,氧化石墨烯的浓度依赖性团聚的潜在影响进行了讨论。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects on digestive enzyme activities in the house crickets Acheta domesticus exposed to graphene oxide in food for several generations.

Increasing usage of nanoparticles or nanomaterials may lead to their release into the environment. The toxicity of these structures, classified as contaminants of emerging concern, is not yet sufficiently understood. However, as in the case of other environmental stressors, the effects of exposure to them should be analyzed on a multigenerational scale to predict the consequences for exposed populations. Therefore, this project aimed to assess the impact of graphene oxide (GO) nanomaterial on digestive enzyme activities in the house cricket Acheta domesticus as a model species, depending on GO concentration (0.2 or 0.02 µg·g-1 dry weight of food), previous selection for longevity and the number of generations (1-5) that have occurred since the beginning of exposure. The last and sixth generations were insects for which GO was withdrawn from the diet (recovery generation). Enzymatic activity was tested using API Zym kit modified for spectrophotometric reads. The tests revealed that GO intervenes with some digestive enzymes. Moreover, the effects of GO depend on the population's previous selection for longevity. The impact of mechanisms mitigating the consequences of aging supports the possible tolerance to GO intoxication. It demonstrated itself in diverse patterns of multigenerational response to GO in wild and long-lived insects. Also, multigenerational exposure revealed the 'third generation' effect. Finally, the impact of GO elimination depended on the concentration of nanomaterial used for the tests. Also, the potential impact of concentration-dependent agglomeration of GO in the context of hormesis has been discussed.

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来源期刊
Nanotoxicology
Nanotoxicology 医学-毒理学
CiteScore
10.10
自引率
4.00%
发文量
45
审稿时长
3.5 months
期刊介绍: Nanotoxicology invites contributions addressing research relating to the potential for human and environmental exposure, hazard and risk associated with the use and development of nano-structured materials. In this context, the term nano-structured materials has a broad definition, including ‘materials with at least one dimension in the nanometer size range’. These nanomaterials range from nanoparticles and nanomedicines, to nano-surfaces of larger materials and composite materials. The range of nanomaterials in use and under development is extremely diverse, so this journal includes a range of materials generated for purposeful delivery into the body (food, medicines, diagnostics and prosthetics), to consumer products (e.g. paints, cosmetics, electronics and clothing), and particles designed for environmental applications (e.g. remediation). It is the nano-size range if these materials which unifies them and defines the scope of Nanotoxicology . While the term ‘toxicology’ indicates risk, the journal Nanotoxicology also aims to encompass studies that enhance safety during the production, use and disposal of nanomaterials. Well-controlled studies demonstrating a lack of exposure, hazard or risk associated with nanomaterials, or studies aiming to improve biocompatibility are welcomed and encouraged, as such studies will lead to an advancement of nanotechnology. Furthermore, many nanoparticles are developed with the intention to improve human health (e.g. antimicrobial agents), and again, such articles are encouraged. In order to promote quality, Nanotoxicology will prioritise publications that have demonstrated characterisation of the nanomaterials investigated.
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