SWEET15糖转运基因突变影响柑橘对黄龙冰病和溃疡病的反应

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Archana Khadgi, Omar Zayed, Cintia H D Sagawa, Fei Zhang, Danelle K Seymour, Vivian F Irish
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引用次数: 0

摘要

黄龙病、柑橘溃疡病等细菌性病害严重影响柑橘生产。由“亚洲解放候选菌”(Candidatus Liberibacter asiaticus, CLas)引起的HLB导致树木衰退,而由柑橘黄单胞菌(Xanthomonas citri pv.)引起的柑橘溃疡病。柑橘(Xcc)会导致叶子和果实的坏死。许多细菌病原体分泌效应蛋白,通过上调宿主编码的易感基因来抑制宿主植物免疫并促进发病。Xcc利用III型分泌系统引入转录因子样(transcription factor-like, TAL)效应物PthA4等效应蛋白,可直接激活宿主易感基因表达。相比之下,CLas缺乏大多数细菌分泌系统,主要依靠Sec分泌系统发病。虽然在CLas中发现了一些sec分泌蛋白,但它们在引起HLB症状中的直接作用仍未得到证实。编码蔗糖转运蛋白的SWEET基因是候选的易感基因。本文研究了SWEET10、SWEET12和SWEET15基因的作用,结果表明,SWEET15基因突变导致Carrizo citrange (citrus sinensis ‘Washington’甜橙× Poncirus trifoliata)、Limoneria 8A' Lisbon lemon (citrus limon)和Pineapple甜橙(C. sinensis)对柑橘溃疡病的易感性降低。此外,发生SWEET15突变的里斯本柠檬植株在感染植株中也表现出CLas滴度降低。这些结果表明,SWEET15可能是一种广谱易感基因,破坏SWEET15基因活性可能是一种可行的方法,可以减轻各种柑橘品种的细菌性疾病,如柑橘溃疡病和HLB。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mutations in the SWEET15 Sugar Transporter Gene Affect Response of Citrus to Huanglongbing Disease and Citrus Canker.

Bacterial diseases like huanglongbing (HLB) and citrus canker severely impact citrus production. HLB, caused by "Candidatus Liberibacter asiaticus" (CLas), leads to tree decline, while citrus canker, caused by Xanthomonas citri pv. citri (Xcc) causes necrotic lesions on leaves and fruit. Many bacterial pathogens secrete effector proteins that suppress host plant immunity and promote pathogenesis through the upregulation of host-encoded susceptibility genes. Xcc uses the type III secretion system to introduce effector proteins such as the transcription factor-like (TAL) effector PthA4 that can directly activate host susceptibility gene expression. In contrast, CLas lacks most bacterial secretion systems and relies predominantly on the Sec secretion system for pathogenesis. While some Sec-secreted proteins have been identified in CLas, their direct role in causing HLB symptoms remains unproven. Several Sugars Will Eventually be Exported Transporter (SWEET) genes, encoding sucrose transporters, are candidate susceptibility genes. Here we investigate the roles of the citrus SWEET10, SWEET12 and SWEET15 genes and show that mutations of SWEET15 resulted in reduced susceptibility to citrus canker in three different citrus cultivars: Carrizo citrange (Citrus sinensis 'Washington' sweet orange × Poncirus trifoliata), 'Limoneria 8A' Lisbon lemon (Citrus limon) and 'Pineapple' sweet orange (C. sinensis). Furthermore, Lisbon lemon plants mutated for SWEET15 also showed reduced CLas titre in infected plants. These results suggest that SWEET15 may act as a broad-spectrum susceptibility gene, and disruption of SWEET15 gene activity could be a viable approach to mitigating bacterial diseases such as citrus canker and HLB in a variety of citrus cultivars.

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来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
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