作为酪氨酸酶抑制剂的天然模拟4,6-二羟基脲酮衍生物:设计、合成和生物学评价。

IF 1.9 4区 医学 Q3 CHEMISTRY, MEDICINAL
Quoc-Thai Nguyen, Giao Quynh Tran, Huy Thanh Ta, Quang Dang Do, Quynh Xuan Vu, Bich-Loan T Phung, Thanh-Dao Tran, Khac-Minh Thai, Cam-Van T Vo
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引用次数: 0

摘要

简介:酪氨酸酶是黑色素生物合成和食物褐变的关键酶,已成为抑制剂开发的重要靶点。目的:研究具有不同B环取代基的4,6-二羟脲酮衍生物对蘑菇酪氨酸酶的抑制作用。方法:设计了20个衍生物并进行了计算研究,揭示了它们与酶活性位点结合并与关键残基和铜离子相互作用的潜力。结果:体外紫外-可见分光光度法测定结果表明,含3,4-二氯苯基团的化合物5h对黄曲酸的抑制活性最高(IC50 = 6.3±0.3 μM), IC50 = 136.5±11.5 μM;进一步的动力学分析和对接模拟表明,5h通过混合抑制机制运行,竞争和非竞争抑制常数分别为21 μM和68 μM。结论:这些发现突出了4,6-二羟基脲酮衍生物作为酪氨酸酶抑制剂在制药、化妆品和农业中的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Natural Mimetic 4,6-dihydroxyaurone Derivatives as Tyrosinase Inhibitors: Design, Synthesis, and Biological Evaluation.

Introduction: Tyrosinase, a key enzyme in melanin biosynthesis and food browning, has become an important target for inhibitor development.

Aim: This study aimed to investigate the inhibitory potential of 4,6-dihydroxyaurone derivatives with varied ring B substituents on mushroom tyrosinase.

Method: Twenty derivatives were designed and subjected to computational studies, revealing their potential to bind to the enzyme's active site and interact with key residues and copper ions.

Result: In vitro UV-Vis spectrophotometry assays on these twenty synthesized aurones demonstrated that compound 5h, featuring a 3,4-dichlorophenyl group at ring B, exhibited the most potent inhibitory activity (IC50 = 6.3 ± 0.3 μM) compared to kojic acid (IC50 = 136.5 ± 11.5 μM). Further kinetic analysis and docking simulations suggested that 5h operated via a mixed inhibition mechanism with competitive and uncompetitive inhibitory constants of 21 μM and 68 μM, respectively.

Conclusion: These findings highlight the promising potential of 4,6-dihydroxyaurone derivatives as potent tyrosinase inhibitors for applications in pharmaceuticals, cosmetics, and agriculture.

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来源期刊
Medicinal Chemistry
Medicinal Chemistry 医学-医药化学
CiteScore
4.30
自引率
4.30%
发文量
109
审稿时长
12 months
期刊介绍: Aims & Scope Medicinal Chemistry a peer-reviewed journal, aims to cover all the latest outstanding developments in medicinal chemistry and rational drug design. The journal publishes original research, mini-review articles and guest edited thematic issues covering recent research and developments in the field. Articles are published rapidly by taking full advantage of Internet technology for both the submission and peer review of manuscripts. Medicinal Chemistry is an essential journal for all involved in drug design and discovery.
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