Quoc-Thai Nguyen, Giao Quynh Tran, Huy Thanh Ta, Quang Dang Do, Quynh Xuan Vu, Bich-Loan T Phung, Thanh-Dao Tran, Khac-Minh Thai, Cam-Van T Vo
{"title":"作为酪氨酸酶抑制剂的天然模拟4,6-二羟基脲酮衍生物:设计、合成和生物学评价。","authors":"Quoc-Thai Nguyen, Giao Quynh Tran, Huy Thanh Ta, Quang Dang Do, Quynh Xuan Vu, Bich-Loan T Phung, Thanh-Dao Tran, Khac-Minh Thai, Cam-Van T Vo","doi":"10.2174/0115734064371213250508115259","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Tyrosinase, a key enzyme in melanin biosynthesis and food browning, has become an important target for inhibitor development.</p><p><strong>Aim: </strong>This study aimed to investigate the inhibitory potential of 4,6-dihydroxyaurone derivatives with varied ring B substituents on mushroom tyrosinase.</p><p><strong>Method: </strong>Twenty derivatives were designed and subjected to computational studies, revealing their potential to bind to the enzyme's active site and interact with key residues and copper ions.</p><p><strong>Result: </strong>In vitro UV-Vis spectrophotometry assays on these twenty synthesized aurones demonstrated that compound 5h, featuring a 3,4-dichlorophenyl group at ring B, exhibited the most potent inhibitory activity (IC<sub>50</sub> = 6.3 ± 0.3 μM) compared to kojic acid (IC<sub>50</sub> = 136.5 ± 11.5 μM). Further kinetic analysis and docking simulations suggested that 5h operated via a mixed inhibition mechanism with competitive and uncompetitive inhibitory constants of 21 μM and 68 μM, respectively.</p><p><strong>Conclusion: </strong>These findings highlight the promising potential of 4,6-dihydroxyaurone derivatives as potent tyrosinase inhibitors for applications in pharmaceuticals, cosmetics, and agriculture.</p>","PeriodicalId":18382,"journal":{"name":"Medicinal Chemistry","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Natural Mimetic 4,6-dihydroxyaurone Derivatives as Tyrosinase Inhibitors: Design, Synthesis, and Biological Evaluation.\",\"authors\":\"Quoc-Thai Nguyen, Giao Quynh Tran, Huy Thanh Ta, Quang Dang Do, Quynh Xuan Vu, Bich-Loan T Phung, Thanh-Dao Tran, Khac-Minh Thai, Cam-Van T Vo\",\"doi\":\"10.2174/0115734064371213250508115259\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Tyrosinase, a key enzyme in melanin biosynthesis and food browning, has become an important target for inhibitor development.</p><p><strong>Aim: </strong>This study aimed to investigate the inhibitory potential of 4,6-dihydroxyaurone derivatives with varied ring B substituents on mushroom tyrosinase.</p><p><strong>Method: </strong>Twenty derivatives were designed and subjected to computational studies, revealing their potential to bind to the enzyme's active site and interact with key residues and copper ions.</p><p><strong>Result: </strong>In vitro UV-Vis spectrophotometry assays on these twenty synthesized aurones demonstrated that compound 5h, featuring a 3,4-dichlorophenyl group at ring B, exhibited the most potent inhibitory activity (IC<sub>50</sub> = 6.3 ± 0.3 μM) compared to kojic acid (IC<sub>50</sub> = 136.5 ± 11.5 μM). Further kinetic analysis and docking simulations suggested that 5h operated via a mixed inhibition mechanism with competitive and uncompetitive inhibitory constants of 21 μM and 68 μM, respectively.</p><p><strong>Conclusion: </strong>These findings highlight the promising potential of 4,6-dihydroxyaurone derivatives as potent tyrosinase inhibitors for applications in pharmaceuticals, cosmetics, and agriculture.</p>\",\"PeriodicalId\":18382,\"journal\":{\"name\":\"Medicinal Chemistry\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2025-05-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medicinal Chemistry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.2174/0115734064371213250508115259\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MEDICINAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medicinal Chemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.2174/0115734064371213250508115259","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
Natural Mimetic 4,6-dihydroxyaurone Derivatives as Tyrosinase Inhibitors: Design, Synthesis, and Biological Evaluation.
Introduction: Tyrosinase, a key enzyme in melanin biosynthesis and food browning, has become an important target for inhibitor development.
Aim: This study aimed to investigate the inhibitory potential of 4,6-dihydroxyaurone derivatives with varied ring B substituents on mushroom tyrosinase.
Method: Twenty derivatives were designed and subjected to computational studies, revealing their potential to bind to the enzyme's active site and interact with key residues and copper ions.
Result: In vitro UV-Vis spectrophotometry assays on these twenty synthesized aurones demonstrated that compound 5h, featuring a 3,4-dichlorophenyl group at ring B, exhibited the most potent inhibitory activity (IC50 = 6.3 ± 0.3 μM) compared to kojic acid (IC50 = 136.5 ± 11.5 μM). Further kinetic analysis and docking simulations suggested that 5h operated via a mixed inhibition mechanism with competitive and uncompetitive inhibitory constants of 21 μM and 68 μM, respectively.
Conclusion: These findings highlight the promising potential of 4,6-dihydroxyaurone derivatives as potent tyrosinase inhibitors for applications in pharmaceuticals, cosmetics, and agriculture.
期刊介绍:
Aims & Scope
Medicinal Chemistry a peer-reviewed journal, aims to cover all the latest outstanding developments in medicinal chemistry and rational drug design. The journal publishes original research, mini-review articles and guest edited thematic issues covering recent research and developments in the field. Articles are published rapidly by taking full advantage of Internet technology for both the submission and peer review of manuscripts. Medicinal Chemistry is an essential journal for all involved in drug design and discovery.