CSF1-CAR特异性靶向CSF1R+胰腺癌细胞和肿瘤相关巨噬细胞

IF 3.2 4区医学 Q3 IMMUNOLOGY

Journal of Immunotherapy Pub Date : 2025-05-16 DOI:10.1097/CJI.0000000000000563

Yongjie Zhu, Ruipu Sun, Jiawei Fan, Haiyan Ma, Bin Sun

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引用次数: 0

摘要

摘要：高度抑制性肿瘤免疫微环境和非特异性靶点赋予恶性肿瘤CAR-T细胞。与GEPIA数据库中的正常组织相比，CSF1R在胰腺癌组织和主要参与抑制肿瘤微环境（suppressive tumor microenvironment， TME）的M2巨噬细胞中表达较高，提示CSF1R是一种合适的抗原。CSF1是CSF1R的天然配体，因此我们构建了CSF1- car，并在体外测试了其对肿瘤细胞和巨噬细胞的细胞毒作用。我们的研究结果表明，CSF1-CAR-T细胞可以依赖于CSF1R的表达来裂解肿瘤细胞。同时，CSF1-CAR-T还能裂解CSF1R+ M2巨噬细胞，提示CSF1-CAR-T细胞具有清除肿瘤细胞、重塑TME的作用。

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CSF1-CAR Specifically Targets CSF1R+ Pancreatic Cancer Cells and Tumor-Associated Macrophages.

Summary: A highly suppressive tumor immune microenvironment and nonspecific target endow malignant tumors with CAR-T cells. CSF1R is highly expressed on pancreatic cancer tissues compares with normal tissues in GEPIA database and M2 macrophages mainly contributing to the suppressive tumor microenvironment (TME), suggesting that CSF1R is a suitable antigen. CSF1 is the natural ligand of CSF1R, so we constructed a CSF1-CAR and tested its cytotoxic effect on tumor cells and macrophages in vitro. Our results demonstrated that CSF1-CAR-T cells can lyse tumor cells dependent on CSF1R expression. Meanwhile, CSF1-CAR-T also lyse CSF1R+ M2 macrophages, suggesting that CSF1-CAR-T cells play a role in eliminating tumor cells and remodeling the TME.

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来源期刊

Journal of Immunotherapy 医学-免疫学

CiteScore

6.90

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Journal of Immunotherapy features rapid publication of articles on immunomodulators, lymphokines, antibodies, cells, and cell products in cancer biology and therapy. Laboratory and preclinical studies, as well as investigative clinical reports, are presented. The journal emphasizes basic mechanisms and methods for the rapid transfer of technology from the laboratory to the clinic. JIT contains full-length articles, review articles, and short communications.