NCAPG2促进胃腺癌细胞的增殖、转移和对nab-紫杉醇的耐药性。

IF 1.9 4区医学 Q3 INFECTIOUS DISEASES

Journal of Chemotherapy Pub Date : 2025-05-15 DOI:10.1080/1120009X.2025.2498230

Jia Xu, Xiaoyuan Liu, Zebo Huang, Ying Zhang, Xia Li, Dongyan Cai

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引用次数: 0

摘要

胃腺癌是世界上最常见的癌症之一。本研究旨在探讨非平滑肌细胞聚集蛋白II复合物G2亚基（NCAPG2）在胃癌细胞中的作用。采用细胞计数试剂盒-8、流式细胞术、Transwell室和裸鼠异种移植模型检测细胞生长、增殖、迁移、侵袭及白蛋白结合紫杉醇耐药性。Western blot检测NCAPG2、STAT3、NF-κB信号通路的表达情况。NCAPG2在胃腺癌细胞中的表达明显上调。敲低NCAPG2可抑制细胞增殖、迁移和侵袭，抑制肿瘤生长。此外，NCAPG2的下调降低了胃腺癌细胞对白蛋白结合紫杉醇的耐药性，下调了STAT3和NF-κB信号通路。综上所述，NCAPG2在胃腺癌的恶性进展中发挥重要作用，并参与白蛋白结合紫杉醇的耐药。

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NCAPG2 promotes the proliferation, metastasis and resistance to nab-paclitaxel in gastric adenocarcinoma cells.

Gastric adenocarcinoma is one of the most common cancers in the world. The purpose of this study was to investigate the role of non-smooth muscle cell aggregation protein II complex G2 subunit (NCAPG2) in gastric cancer cells. Cell growth, proliferation, migration, invasion and albumin-bound paclitaxel resistance were detected by cell counting kit-8, flow cytometry, Transwell chamber and nude mouse xenograft model. Western blot was used to detect the expression of NCAPG2, STAT3 and NF-κB signaling pathways. The expression of NCAPG2 was significantly up-regulated in gastric adenocarcinoma cells. Knockdown of NCAPG2 inhibited cell proliferation, migration and invasion, and inhibited tumor growth. In addition, knockdown of NCAPG2 reduced the resistance of gastric adenocarcinoma cells to albumin-bound paclitaxel and down-regulated STAT3 and NF-κB signaling pathways. In summary, NCAPG2 plays an important role in the malignant progression of gastric adenocarcinoma and is involved in the resistance to albumin-bound paclitaxel.

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来源期刊

Journal of Chemotherapy 医学-药学

CiteScore

3.70

自引率

0.00%

发文量

144

审稿时长

6-12 weeks

期刊介绍： The Journal of Chemotherapy is an international multidisciplinary journal committed to the rapid publication of high quality, peer-reviewed, original research on all aspects of antimicrobial and antitumor chemotherapy. The Journal publishes original experimental and clinical research articles, state-of-the-art reviews, brief communications and letters on all aspects of chemotherapy, providing coverage of the pathogenesis, diagnosis, treatment, and control of infection, as well as the use of anticancer and immunomodulating drugs. Specific areas of focus include, but are not limited to: · Antibacterial, antiviral, antifungal, antiparasitic, and antiprotozoal agents; · Anticancer classical and targeted chemotherapeutic agents, biological agents, hormonal drugs, immunomodulatory drugs, cell therapy and gene therapy; · Pharmacokinetic and pharmacodynamic properties of antimicrobial and anticancer agents; · The efficacy, safety and toxicology profiles of antimicrobial and anticancer drugs; · Drug interactions in single or combined applications; · Drug resistance to antimicrobial and anticancer drugs; · Research and development of novel antimicrobial and anticancer drugs, including preclinical, translational and clinical research; · Biomarkers of sensitivity and/or resistance for antimicrobial and anticancer drugs; · Pharmacogenetics and pharmacogenomics; · Precision medicine in infectious disease therapy and in cancer therapy; · Pharmacoeconomics of antimicrobial and anticancer therapies and the implications to patients, health services, and the pharmaceutical industry.