紫外可见光谱法检测Fe3 +离子ph依赖碳点灵敏度的多变量分析。

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS
Muna E Raypah, Mohd Faizal Jamlos, Jelena Muncan, Ku Muhammad Naim Ku Khalif, Sarah Atifah Saruchi, Munira Mohd Ali, Chin Sim Yee, Agus Nugroho, Prabowo Puranto
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引用次数: 0

摘要

近年来,荧光碳点(CDs)已成为一种有效的、适应性强的纳米材料,可用于重金属离子的灵敏和特异检测。铁离子(Fe3+)是能渗入人体和环境的最具破坏性的金属离子之一。本研究以柠檬汁为原料,采用水热法制备了蓝色发光碳点(CDs)。在不同的pH条件下(3、5、7、9、11和13),使用不同浓度的Fe3+(0-400µM),通过测量200-700 nm处的UV-Vis吸光度来检测CDs对Fe3+离子的敏感性。结果表明,CDs与Fe3+的相互作用受pH的显著影响,导致不同的吸光度增强响应。为了更深入地了解这种与ph相关的性能,我们使用了多变量分析技术,包括主成分分析(PCA)、线性判别分析(LDA)和偏最小二乘回归(PLSR)。通过将这些技术与实验数据相结合,确定了pH水平、CDs的π - π∗和n - π∗电子跃迁和Fe3⁺传感性能之间的显著相关性。根据PLSR模型,pH 7是现实应用的理想选择,因为它提供了Fe3⁺溶解度和CDs传感能力之间的最佳平衡,与环境和生理条件密切匹配。这项工作有助于了解基于cd的传感机制,并强调了多元分析在提高生物化学和环境领域实际应用中的材料性能方面的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multivariate Analysis of pH-Dependent Carbon Dots Sensitivity for Fe3⁺ Ions Detection Using UV-VIS Spectroscopy.

Fluorescent carbon dots (CDs) have become a potent and adaptable nanomaterial in recent years for the sensitive and specific detection of heavy metal ions. Ferric ion (Fe3+) is one of the most damaging metal ions that can infiltrate the human body and the environment. In this study, blue-emitting carbon dots (CDs) were successfully synthesized from lemon juice using a hydrothermal process. The sensitivity of CDs to Fe3+ ions was examined using various concentrations of Fe3+ (0-400 µM) under different pH conditions (3, 5, 7, 9, 11, and 13) by measuring UV-Vis absorbance at 200-700 nm. The findings showed that the interaction between CDs and Fe3+ is significantly influenced by pH, resulting in varying absorbance enhancement responses. To get more profound insights into this pH-dependent performance, multivariate analysis techniques, including principal component analysis (PCA), linear discriminant analysis (LDA), and partial least squares regression (PLSR) were utilized. By combining these techniques with experimental data, significant correlations between pH levels, π - π and n - π electronic transitions of CDs, and Fe3⁺ sensing performance were identified. According to the PLSR model, pH 7 is ideal for real-world uses since it offers the optimum balance between Fe3⁺ solubility and CDs sensing capabilities, closely matching environmental and physiological conditions. This work contributes to the knowledge of CDs-based sensing mechanisms and emphasizes the value of multivariate analysis in boosting material performance for real-world applications in the biochemical and environmental domains.

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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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