结肠活检标本中巨细胞病毒免疫组化染色阳性提示qPCR中病毒DNA载量高。

IF 0.9 4区 医学 Q4 PATHOLOGY
Guanglan Wang, Jianmin Zhao, Junchang Jiang, Aihua Huang, Lingna Ye, Zhinong Jiang
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引用次数: 0

摘要

背景和目的免疫组织化学(IHC)和/或聚合酶链反应(PCR)是确认炎症性肠病(IBD)患者巨细胞病毒(CMV)活动性感染的必要方法。尚未确定一个明确的截止日期;然而,Roblin等人认为病毒载量阈值为每毫克组织250个病毒拷贝。在这项研究中,我们检验了免疫组化和实时荧光定量PCR (qPCR)检测结肠活检标本中巨细胞病毒的一致性。方法采用免疫组化(IHC)和qPCR方法采集标本170份。研究IHC和qPCR的阳性率和一致性,并观察溃疡组织对两种检测方法的影响。结果对170例苏木精伊红(H&E)切片进行分析,发现8例巨细胞病毒感染。免疫组化和qPCR检测CMV阳性率分别为17%(29/170)和25%(43/170)。在ihc阳性样本中,qPCR阳性率为100%(29/29),所有CMV-DNA载量均为250拷贝/mg。IHC与qPCR的一致性为94% (kappa = 0.756, P = 250 copies/mg)。与未溃疡的粘膜相比,溃疡粘膜中ihc阳性细胞更常见。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Positive Immunohistochemical Staining of Cytomegalovirus in Colon Biopsy Samples Suggests That the Viral DNA Load in qPCR Is High.

Background and AimImmunohistochemistry (IHC) and/or polymerase chain reaction (PCR) are essential for confirming active cytomegalovirus (CMV) infection in patients with inflammatory bowel disease (IBD). A definitive cut-off has not yet been established; however, Roblin et al suggested a viral load threshold of > 250 viral copies/mg of tissue. In this study, we examined the concordance between IHC and quantitative real-time PCR (qPCR) in the detection of CMV in colonic biopsy specimens.MethodsA total of 170 samples that underwent IHC and qPCR were collected. The positive rate and concordance of IHC and qPCR were studied, and the impact of ulcer tissue on both detection methods was observed.ResultsIn a study of 170 hematoxylin-eosin (H&E) sections, 8 samples were found to be infected with cytomegalovirus. The positive rates of CMV detection using IHC and qPCR were 17% (29/170) and 25% (43/170), respectively. Among the IHC-positive samples, the qPCR positive rate was 100% (29/29), with all CMV-DNA loads > 250 copies/mg. The concordance between IHC and qPCR was 94% (kappa = 0.756, P < .001). IHC analysis revealed that positive cells in ulcerated mucosa were more frequent than those in nonulcerated mucosa (P < .001). Additionally, a total of 14 samples tested negative for CMV via IHC but positive through qPCR. Among these, 11 samples did not contain ulcerated tissue.ConclusionThe presence of one or more IHC-positive cells in colon biopsy samples indicates a high CMV-DNA load in qPCR (> 250 copies/mg). IHC-positive cells were more frequently observed in ulcerated mucosa compared to nonulcerated mucosa.

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来源期刊
CiteScore
2.10
自引率
0.00%
发文量
198
审稿时长
1 months
期刊介绍: International Journal of Surgical Pathology (IJSP) is a peer-reviewed journal published eight times a year, which offers original research and observations covering all major organ systems, timely reviews of new techniques and procedures, discussions of controversies in surgical pathology, case reports, and images in pathology. This journal is a member of the Committee on Publication Ethics (COPE).
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