{"title":"Lnc MSTRG 4701.7靶向miR-1786/RORa竞争性调节鸡滤泡颗粒细胞的增殖和凋亡。","authors":"Chang Ma, Hengsong Wu, Chunchi Yan, Simushi Liswaniso, Xue Sun, Ning Qin, Rifu Xu","doi":"10.3389/fvets.2025.1583287","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Follicular development in chickens is a highly coordinated and complex process. While the hypothalamic-pituitary-gonadal axis plays a central regulatory role in this process, the role of long noncoding RNAs (LncRNAs) remains unclear. Here, we aimed to identify key differentially expressed LncRNAs and explore their regulatory roles in follicular development, particularly focusing on the MSTRG.4701.7-miR-1786-RORa axis, to provide insights for improving fertility in low-fertility chicken varieties.</p><p><strong>Methods: </strong>Intact follicular tissues were collected from Jilin Black chickens (low-fertility group) and Lohmann Brown Laying hens (high-fertility group) at each stage of ovarian development for transcriptome sequencing to identify key differentially expressed LncRNAs for follow-up analyses. Bioinformatics analysis was also performed to determine the role of the MSTRG.4701.7-miR-1786-RORa regulatory axis, and to clarify whether that MSTRG 4701.7 targets miR-1786/RORa to competitively regulate the proliferation and differentiation of chicken follicular granulosa cells (GCs). We also knocked down and overexpressed key genes, MSTRG.4701.7 and miR-1786 and thereafter, determined changes in the mRNA and protein expression levels of axial terminal mRNA-RORa.</p><p><strong>Results: </strong>MSTRG.4701.7 promotes the apoptosis of chicken follicular GCs, while miR-1786 reverses this phenomenon. RORa was also identified as a functional target of miR-1786 in GCs, and as a competitive endogenous RNA, MSTRG.4701.7 regulated RORa expression by sponging miR-1786, thereby playing a regulatory role in GCs. Additionally, interfering with MSTRG.4701.7 expression significantly downregulated RORa mRNA and protein expression levels, while interfering with miR-1786 showed the opposite effect.</p><p><strong>Conclusion: </strong>MSTRG.4701.7 plays a critical role in follicular development in chickens by influencing GC proliferation, differentiation, and apoptosis. The resent results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.</p>","PeriodicalId":12772,"journal":{"name":"Frontiers in Veterinary Science","volume":"12 ","pages":"1583287"},"PeriodicalIF":2.6000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075847/pdf/","citationCount":"0","resultStr":"{\"title\":\"Lnc MSTRG 4701.7 targets miR-1786/RORa to competitively regulate proliferation and apoptosis in chicken follicular granulosa cells.\",\"authors\":\"Chang Ma, Hengsong Wu, Chunchi Yan, Simushi Liswaniso, Xue Sun, Ning Qin, Rifu Xu\",\"doi\":\"10.3389/fvets.2025.1583287\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Follicular development in chickens is a highly coordinated and complex process. While the hypothalamic-pituitary-gonadal axis plays a central regulatory role in this process, the role of long noncoding RNAs (LncRNAs) remains unclear. Here, we aimed to identify key differentially expressed LncRNAs and explore their regulatory roles in follicular development, particularly focusing on the MSTRG.4701.7-miR-1786-RORa axis, to provide insights for improving fertility in low-fertility chicken varieties.</p><p><strong>Methods: </strong>Intact follicular tissues were collected from Jilin Black chickens (low-fertility group) and Lohmann Brown Laying hens (high-fertility group) at each stage of ovarian development for transcriptome sequencing to identify key differentially expressed LncRNAs for follow-up analyses. Bioinformatics analysis was also performed to determine the role of the MSTRG.4701.7-miR-1786-RORa regulatory axis, and to clarify whether that MSTRG 4701.7 targets miR-1786/RORa to competitively regulate the proliferation and differentiation of chicken follicular granulosa cells (GCs). We also knocked down and overexpressed key genes, MSTRG.4701.7 and miR-1786 and thereafter, determined changes in the mRNA and protein expression levels of axial terminal mRNA-RORa.</p><p><strong>Results: </strong>MSTRG.4701.7 promotes the apoptosis of chicken follicular GCs, while miR-1786 reverses this phenomenon. RORa was also identified as a functional target of miR-1786 in GCs, and as a competitive endogenous RNA, MSTRG.4701.7 regulated RORa expression by sponging miR-1786, thereby playing a regulatory role in GCs. Additionally, interfering with MSTRG.4701.7 expression significantly downregulated RORa mRNA and protein expression levels, while interfering with miR-1786 showed the opposite effect.</p><p><strong>Conclusion: </strong>MSTRG.4701.7 plays a critical role in follicular development in chickens by influencing GC proliferation, differentiation, and apoptosis. The resent results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.</p>\",\"PeriodicalId\":12772,\"journal\":{\"name\":\"Frontiers in Veterinary Science\",\"volume\":\"12 \",\"pages\":\"1583287\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12075847/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3389/fvets.2025.1583287\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q1\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3389/fvets.2025.1583287","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Lnc MSTRG 4701.7 targets miR-1786/RORa to competitively regulate proliferation and apoptosis in chicken follicular granulosa cells.
Background: Follicular development in chickens is a highly coordinated and complex process. While the hypothalamic-pituitary-gonadal axis plays a central regulatory role in this process, the role of long noncoding RNAs (LncRNAs) remains unclear. Here, we aimed to identify key differentially expressed LncRNAs and explore their regulatory roles in follicular development, particularly focusing on the MSTRG.4701.7-miR-1786-RORa axis, to provide insights for improving fertility in low-fertility chicken varieties.
Methods: Intact follicular tissues were collected from Jilin Black chickens (low-fertility group) and Lohmann Brown Laying hens (high-fertility group) at each stage of ovarian development for transcriptome sequencing to identify key differentially expressed LncRNAs for follow-up analyses. Bioinformatics analysis was also performed to determine the role of the MSTRG.4701.7-miR-1786-RORa regulatory axis, and to clarify whether that MSTRG 4701.7 targets miR-1786/RORa to competitively regulate the proliferation and differentiation of chicken follicular granulosa cells (GCs). We also knocked down and overexpressed key genes, MSTRG.4701.7 and miR-1786 and thereafter, determined changes in the mRNA and protein expression levels of axial terminal mRNA-RORa.
Results: MSTRG.4701.7 promotes the apoptosis of chicken follicular GCs, while miR-1786 reverses this phenomenon. RORa was also identified as a functional target of miR-1786 in GCs, and as a competitive endogenous RNA, MSTRG.4701.7 regulated RORa expression by sponging miR-1786, thereby playing a regulatory role in GCs. Additionally, interfering with MSTRG.4701.7 expression significantly downregulated RORa mRNA and protein expression levels, while interfering with miR-1786 showed the opposite effect.
Conclusion: MSTRG.4701.7 plays a critical role in follicular development in chickens by influencing GC proliferation, differentiation, and apoptosis. The resent results provide useful molecular evidence for elucidating the genetic mechanism underlying ovarian follicle development associated with egg production in chicken.
期刊介绍：
Frontiers in Veterinary Science is a global, peer-reviewed, Open Access journal that bridges animal and human health, brings a comparative approach to medical and surgical challenges, and advances innovative biotechnology and therapy.
Veterinary research today is interdisciplinary, collaborative, and socially relevant, transforming how we understand and investigate animal health and disease. Fundamental research in emerging infectious diseases, predictive genomics, stem cell therapy, and translational modelling is grounded within the integrative social context of public and environmental health, wildlife conservation, novel biomarkers, societal well-being, and cutting-edge clinical practice and specialization. Frontiers in Veterinary Science brings a 21st-century approach—networked, collaborative, and Open Access—to communicate this progress and innovation to both the specialist and to the wider audience of readers in the field.
Frontiers in Veterinary Science publishes articles on outstanding discoveries across a wide spectrum of translational, foundational, and clinical research. The journal''s mission is to bring all relevant veterinary sciences together on a single platform with the goal of improving animal and human health.
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