Elsayed M Younis, Rowida E Ibrahim, Abdelwahab A Abdelwarith, Engy Mohamed Mohamed Yassin, Kholoud A Alhallag, Mohammed S Sobh, Mohamed Shaalan, Tarek Khamis, Afaf N Abdel Rahman
{"title":"小叶Malva parviflora叶片对尼罗罗非鱼Polyram DF(干流)毒性的缓解潜力:免疫/凋亡标志物,生化,抗氧化和组织病理学见解。","authors":"Elsayed M Younis, Rowida E Ibrahim, Abdelwahab A Abdelwarith, Engy Mohamed Mohamed Yassin, Kholoud A Alhallag, Mohammed S Sobh, Mohamed Shaalan, Tarek Khamis, Afaf N Abdel Rahman","doi":"10.1007/s10695-025-01515-w","DOIUrl":null,"url":null,"abstract":"<p><p>Globally, the contamination of aquatic bodies by improper application of pesticides, particularly fungicides, endangered aquatic life and hampered sustainable aquaculture. Exploring innovative strategies to mitigate this difficult issue as eco-friendly and cost-effective is indispensable. Herein, the potential of dietary Egyptian mallow (Malva parviflora) leaves (EML) as a powder form to mitigate the toxicological implications of metiram (MET) exposure in Oreochromis niloticus (Nile tilapia) was assessed in this attempt. The investigation featured growth, physiological, immune/antioxidant responses, splenic cytokines expression, and histopathological concepts. In light of this, fish (N = 200; 32.80 ± 0.92 g) were eventually assigned into four groups with five sets (control, EML, MET, and EML + MET) for 63 days. The control and EML groups were given basal diets that had 0 and 15 g EML/kg diet, respectively, without MET exposure. Furthermore, MET and EML + MET groups were received the identical diets as control and EML groups, respectively, and exposed to 0.38 mg/L MET. The findings highlighted a significant (P < 0.001) growth retardation (declining in weight gain and specific growth rate) and higher fish mortality (32%) by MET exposure. Also, immune impairment (lowered immunoglobulin M and complement 3) and oxidative damage (higher malondialdehyde and declined catalase and superoxide dismutase) were the outcomes of MET toxicity. In addition, the biochemical biomarkers showed substantial disruptions (P < 0.001) in the MET group, where creatinine, aspartate aminotransferase, and alanine aminotransferase increased. Splenic expression of immune-relevant cytokines (interleukin-10 and interferon-gamma 1) was downregulated with upregulation of apoptosis (cysteine-aspartic acid protease-3) linked gene by MET toxicity (P < 0.001). Histologically, MET significantly provoked a diverse range of aberrations in the spleen, kidney, and liver tissues. Remarkably, feeding the EML diet to the MET group decreased fish mortality (14%) and refined all metrics, while retaining the histological integrity of tissues. The conclusion of this viewpoint recommends using dietary EML (15 g/kg) to diminish the deleterious effects of MET in toxicity in Nile tilapia to sustain aquaculture.</p>","PeriodicalId":12274,"journal":{"name":"Fish Physiology and Biochemistry","volume":"51 3","pages":"98"},"PeriodicalIF":2.5000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mitigating potential of Malva parviflora leaves against Polyram DF (dry flowable) toxicity in Nile tilapia: immune/apoptotic markers, biochemical, antioxidant, and histopathological insights.\",\"authors\":\"Elsayed M Younis, Rowida E Ibrahim, Abdelwahab A Abdelwarith, Engy Mohamed Mohamed Yassin, Kholoud A Alhallag, Mohammed S Sobh, Mohamed Shaalan, Tarek Khamis, Afaf N Abdel Rahman\",\"doi\":\"10.1007/s10695-025-01515-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Globally, the contamination of aquatic bodies by improper application of pesticides, particularly fungicides, endangered aquatic life and hampered sustainable aquaculture. Exploring innovative strategies to mitigate this difficult issue as eco-friendly and cost-effective is indispensable. Herein, the potential of dietary Egyptian mallow (Malva parviflora) leaves (EML) as a powder form to mitigate the toxicological implications of metiram (MET) exposure in Oreochromis niloticus (Nile tilapia) was assessed in this attempt. The investigation featured growth, physiological, immune/antioxidant responses, splenic cytokines expression, and histopathological concepts. In light of this, fish (N = 200; 32.80 ± 0.92 g) were eventually assigned into four groups with five sets (control, EML, MET, and EML + MET) for 63 days. The control and EML groups were given basal diets that had 0 and 15 g EML/kg diet, respectively, without MET exposure. Furthermore, MET and EML + MET groups were received the identical diets as control and EML groups, respectively, and exposed to 0.38 mg/L MET. The findings highlighted a significant (P < 0.001) growth retardation (declining in weight gain and specific growth rate) and higher fish mortality (32%) by MET exposure. Also, immune impairment (lowered immunoglobulin M and complement 3) and oxidative damage (higher malondialdehyde and declined catalase and superoxide dismutase) were the outcomes of MET toxicity. In addition, the biochemical biomarkers showed substantial disruptions (P < 0.001) in the MET group, where creatinine, aspartate aminotransferase, and alanine aminotransferase increased. Splenic expression of immune-relevant cytokines (interleukin-10 and interferon-gamma 1) was downregulated with upregulation of apoptosis (cysteine-aspartic acid protease-3) linked gene by MET toxicity (P < 0.001). Histologically, MET significantly provoked a diverse range of aberrations in the spleen, kidney, and liver tissues. Remarkably, feeding the EML diet to the MET group decreased fish mortality (14%) and refined all metrics, while retaining the histological integrity of tissues. The conclusion of this viewpoint recommends using dietary EML (15 g/kg) to diminish the deleterious effects of MET in toxicity in Nile tilapia to sustain aquaculture.</p>\",\"PeriodicalId\":12274,\"journal\":{\"name\":\"Fish Physiology and Biochemistry\",\"volume\":\"51 3\",\"pages\":\"98\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fish Physiology and Biochemistry\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10695-025-01515-w\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish Physiology and Biochemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10695-025-01515-w","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Mitigating potential of Malva parviflora leaves against Polyram DF (dry flowable) toxicity in Nile tilapia: immune/apoptotic markers, biochemical, antioxidant, and histopathological insights.
Globally, the contamination of aquatic bodies by improper application of pesticides, particularly fungicides, endangered aquatic life and hampered sustainable aquaculture. Exploring innovative strategies to mitigate this difficult issue as eco-friendly and cost-effective is indispensable. Herein, the potential of dietary Egyptian mallow (Malva parviflora) leaves (EML) as a powder form to mitigate the toxicological implications of metiram (MET) exposure in Oreochromis niloticus (Nile tilapia) was assessed in this attempt. The investigation featured growth, physiological, immune/antioxidant responses, splenic cytokines expression, and histopathological concepts. In light of this, fish (N = 200; 32.80 ± 0.92 g) were eventually assigned into four groups with five sets (control, EML, MET, and EML + MET) for 63 days. The control and EML groups were given basal diets that had 0 and 15 g EML/kg diet, respectively, without MET exposure. Furthermore, MET and EML + MET groups were received the identical diets as control and EML groups, respectively, and exposed to 0.38 mg/L MET. The findings highlighted a significant (P < 0.001) growth retardation (declining in weight gain and specific growth rate) and higher fish mortality (32%) by MET exposure. Also, immune impairment (lowered immunoglobulin M and complement 3) and oxidative damage (higher malondialdehyde and declined catalase and superoxide dismutase) were the outcomes of MET toxicity. In addition, the biochemical biomarkers showed substantial disruptions (P < 0.001) in the MET group, where creatinine, aspartate aminotransferase, and alanine aminotransferase increased. Splenic expression of immune-relevant cytokines (interleukin-10 and interferon-gamma 1) was downregulated with upregulation of apoptosis (cysteine-aspartic acid protease-3) linked gene by MET toxicity (P < 0.001). Histologically, MET significantly provoked a diverse range of aberrations in the spleen, kidney, and liver tissues. Remarkably, feeding the EML diet to the MET group decreased fish mortality (14%) and refined all metrics, while retaining the histological integrity of tissues. The conclusion of this viewpoint recommends using dietary EML (15 g/kg) to diminish the deleterious effects of MET in toxicity in Nile tilapia to sustain aquaculture.
期刊介绍:
Fish Physiology and Biochemistry is an international journal publishing original research papers in all aspects of the physiology and biochemistry of fishes. Coverage includes experimental work in such topics as biochemistry of organisms, organs, tissues and cells; structure of organs, tissues, cells and organelles related to their function; nutritional, osmotic, ionic, respiratory and excretory homeostasis; nerve and muscle physiology; endocrinology; reproductive physiology; energetics; biochemical and physiological effects of toxicants; molecular biology and biotechnology and more.