结合血清药物化学和网络药理学，探讨仙参方预防运动性疲劳的可能机制。

IF 2.6 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2025-05-13 DOI:10.1016/j.fitote.2025.106621

Qiwen Xuan, Yi Ruan, Zifei Yin, Wei Gu, Changquan Ling

引用次数: 0

摘要

目的：在探讨XSF疗效的基础上，以血吸收成分为研究对象，运用网络药理学方法阐明XSF预防运动性疲劳的物质基础和作用机制。方法：采用UPLC-Q-TOF/MS对XSF血液成分进行鉴定。在此基础上，从Swiss target prediction and SuperPred数据库中获得血液进入成分的靶标预测，从OMIM、GeneCards等疾病数据库中获得运动性疲劳相关靶标。利用Cytoscape软件建立了XSF“组分-靶点-病害”网络模型。利用字符串数据分析平台构建PPI网络，过滤出主要目标。利用DAVID数据库对关键靶点进行基因本体（GO）富集分析和京都基因与基因组百科全书（KEGG）途径富集分析。结果：XSF增加了游泳疲劳大鼠和小鼠的游泳时间，降低了BLA、BUN和MDA水平，升高了SOD水平。共鉴定出60种体外活性成分，45种血液进入原型成分，41种血液进入代谢成分。在717个药物成分与运动衰竭可能的作用靶点中，网络药理学根据PPI分析鉴定出TP53最大值的176个节点（度 = 81）。关键靶点涉及96条KEGG通路，包括PI3K-Akt信号通路、MAPK信号通路、HIF-1信号通路、TNF信号通路和170条GO信号通路。预测XSF抗EIF“组件-目标-通路”网络中的前10个靶点分别为NFKB1、PIK3R1、GRIN1、CACNA1B、SLC6A5、NTRK3、GRIA2、TRIM24、TOP2A、TLR4和RORB、TLR4和RORB。结论：XSF具有预防EIF的作用，其潜在的药理机制可能与改善能量代谢、调节炎症反应、调节氧化应激有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.