一种基于硅膜的双虹膜雕塑若虫人工喂养系统。

IF 1.8 2区 农林科学 Q2 ENTOMOLOGY
Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian
{"title":"一种基于硅膜的双虹膜雕塑若虫人工喂养系统。","authors":"Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian","doi":"10.1007/s10493-025-01026-6","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"94 4","pages":"57"},"PeriodicalIF":1.8000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs.\",\"authors\":\"Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian\",\"doi\":\"10.1007/s10493-025-01026-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.</p>\",\"PeriodicalId\":12088,\"journal\":{\"name\":\"Experimental and Applied Acarology\",\"volume\":\"94 4\",\"pages\":\"57\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental and Applied Acarology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10493-025-01026-6\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Applied Acarology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10493-025-01026-6","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

本研究的目的是建立一种基于硅膜的双虹膜雕塑若虫人工饲养系统(AFS),并将其与传统的实验动物饲养系统进行比较。三组60只若虫分别以兔、小牛和新建立的AFS为食,使用预热(38°C)的脱脂牛血。AFS的附着率(38.3%)和充血率(36.7%)均显著低于对照组(p = 0.0001;P = 0.0002),高于动物性饲养系统(73.3-85%)。在所有三个队列中,充血若虫的后续发育在充血体重(11.96-16.3 mg)和随后的成虫蜕皮(78.3-100%)方面相似。AFS的主要限制是附着和膨胀率低,这需要进一步优化以增强对膜的初始吸引力,例如,通过向膜中添加外部引诱剂或向牛血液中添加ATP等刺激剂。尽管存在这些局限性,但开发的AFS为未来蜱虫、蜱传疾病和药物疗效的研究提供了有价值的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs.

The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.90
自引率
9.10%
发文量
81
审稿时长
4-8 weeks
期刊介绍: Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信