Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian
{"title":"一种基于硅膜的双虹膜雕塑若虫人工喂养系统。","authors":"Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian","doi":"10.1007/s10493-025-01026-6","DOIUrl":null,"url":null,"abstract":"<p><p>The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.</p>","PeriodicalId":12088,"journal":{"name":"Experimental and Applied Acarology","volume":"94 4","pages":"57"},"PeriodicalIF":1.8000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs.\",\"authors\":\"Anna Obiegala, Nina Król, Lara M I Heyse, Martin Pfeffer, Martina Montini, Santiago Nava, Patrick S Sebastian\",\"doi\":\"10.1007/s10493-025-01026-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.</p>\",\"PeriodicalId\":12088,\"journal\":{\"name\":\"Experimental and Applied Acarology\",\"volume\":\"94 4\",\"pages\":\"57\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental and Applied Acarology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1007/s10493-025-01026-6\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENTOMOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental and Applied Acarology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10493-025-01026-6","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
A silicon-membrane based artificial feeding system for Amblyomma sculptum nymphs.
The aim of the study was to establish an artificial feeding system (AFS), based on silicon membranes, for Amblyomma sculptum nymphs and compare it to classical feeding systems using laboratory animals. Three cohorts of 60 nymphs were fed on a rabbit, calf, and with the newly established AFS using prewarmed (38 °C) defibrinated bovine blood. The attachment rate (38.3%) as well as the engorgement rate (36.7%) in the AFS were both significantly lower (p = 0.0001; p = 0.0002) than in the animal-based feeding systems (73.3-85%). Subsequent development of engorged nymphs was similar regarding engorgement weight (11.96-16.3 mg) and subsequent molting (78.3-100%) into adults in all three cohorts. The main limitations of the AFS are the low attachment and engorgement rates, which require further optimization to enhance initial attraction to the membrane, for instance, by adding external attractants to the membrane or stimulating agents such as ATP to the bovine blood. Despite these limitations, the developed AFS provides a valuable tool for future research on ticks, tick-borne diseases and drug efficacy.
期刊介绍:
Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.