505 nm绿色发光二极管抑制黑色素合成的细胞实验及人为干预研究

IF 3.7 4区 医学 Q1 DERMATOLOGY
Yoshihito Mima, Takehiro Yamada, Jun Omatsu, Takashi Yamashita, Shota Suzuki, Takayuki Takechi, Mayumi Ichikawa, Kentaro Yamazaki, Shinichi Sato, Ayumi Yoshizaki
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引用次数: 0

摘要

发光二极管(led)目前因其在治疗色素沉着和皱纹方面的应用而在美容医学中受到关注。据报道,几种波长的LED通过靶向黑色素合成相关的酶、黑色素小体和刺激角质形成细胞黑色素生成的旁分泌因子来抑制黑色素的产生,但绿色LED (GLED)的作用在很大程度上仍未被探索。本研究旨在通过B16黑色素瘤细胞和三维皮肤模型研究GLED对细胞活力、黑色素生成和黑色素相关基因表达的影响。此外,通过配备led的面膜进行人体干预研究,评估其改善皮肤色素沉着的功效。采用B16小鼠黑色素瘤细胞、含黑色素细胞的3D皮肤模型和配备505 nm led面罩的人为干预研究,评估505 nm led对黑色素合成的抑制作用。505 nm的GLED照射通过抑制小眼相关转录因子和酪氨酸酶的基因表达水平,减少黑色素生成诱导的B16小鼠黑色素瘤细胞的黑色素生成。此外,GLED照射也降低了3D皮肤模型中的黑色素含量,但不影响细胞活力。最后,一项人体干预研究表明,照射人体面部皮肤可有效减少色素沉着。这些发现表明505 nm的GLED是一种潜在的脱色策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Inhibitory Effect of 505 nm Green Light Emitting Diode on Melanin Synthesis in Cellular Experiments and a Human Intervention Study.

Light-emitting diodes (LEDs) are currently gaining attention in aesthetic medicine for their applications in the treatment of pigmentation and wrinkles. While several wavelengths of LEDs have been reported to inhibit melanin production by targeting melanin synthesis-related enzymes, melanosomes, and paracrine factors stimulating melanogenesis from keratinocytes, the effects of green LED (GLED) remain largely unexplored. This study aimed to investigate the effects of GLED on cell viability, melanin production, and melanin-related gene expression using B16 melanoma cells and 3-dimensional (3D) skin models. Furthermore, its efficacy in improving skin pigmentation was evaluated through a human intervention study with GLED-equipped facial masks. The melanin synthesis inhibitory effects of 505 nm GLED were evaluated using B16 mouse melanoma cells, a 3D skin model containing melanocytes, and a human intervention study with 505 nm GLED-equipped masks. 505 nm GLED irradiation reduced melanin production in melanogenesis-induced B16 mouse melanoma cells by suppressing the gene expression levels of microphthalmia-associated transcription factor and tyrosinase. Furthermore, GLED irradiation also reduced melanin content in 3D skin models without affecting cell viability. Finally, a human intervention study demonstrated that GLED irradiation on human facial skin effectively decrease pigmentation. These findings suggest 505 nm GLED is a potential depigmentation strategy.

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来源期刊
Acta dermato-venereologica
Acta dermato-venereologica 医学-皮肤病学
CiteScore
4.90
自引率
2.80%
发文量
210
审稿时长
6-12 weeks
期刊介绍: Acta Dermato-Venereologica publishes high-quality manuscripts in English in the field of Dermatology and Venereology, dealing with new observations on basic dermatological and venereological research, as well as clinical investigations. Each volume also features a number of Review articles in special areas, as well as short Letters to the Editor to stimulate debate and to disseminate important clinical observations. Acta Dermato-Venereologica has rapid publication times and is amply illustrated with a large number of colour photographs.
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