{"title":"姜黄素、壳聚糖和钛酸镍纳米杂化物在增强抗菌和抗癌治疗中的协同杀菌作用。","authors":"Ebenezar Jeyasingh, Hendry Moses Panneerselvam, Karthikeyan Chandrasekaran, Sasikumar Moorthy","doi":"10.1021/acsabm.5c00319","DOIUrl":null,"url":null,"abstract":"<p><p>Microbial infections and colorectal cancer are significant contributors to mortality and morbidity, necessitating prompt action. In the present work, NiTiO<sub>3</sub> (NT), curcumin-loaded NiTiO<sub>3</sub> (CUNT), chitosan-loaded NiTiO<sub>3</sub> (CHNT), and both curcumin and chitosan-loaded NiTiO<sub>3</sub> (CUCHNT) hybrid nanomaterials (HNMs) were systematically prepared using <i>Psidium guajava</i> leaf extract. PXRD studies revealed that NT, CUNT, CHNT, and CUCHNT exhibited a rhombohedral structure with the <i>R</i>3̅ space group with mean crystallite sizes of ∼66.41, ∼61.94, ∼61.65, and ∼65.12 nm, respectively. Band gap energies were estimated as ∼3.10, ∼3.15, ∼3.14, and ∼3.15 eV for NT, CUNT, CHNT, and CUCHNT, respectively. Antibacterial efficacies of synthesized HNMs were analyzed against both Gram-positive (G+) and Gram-negative (G-) bacteria using the well diffusion method, and the zone of inhibition (ZOI) exhibited by NT, CUNT, CHNT, and CUCHNT against <i>Staphylococcus aureus</i> were 11.5, 13, 12, and 17.5 mm, respectively; those of <i>Staphylococcus pneumoniae</i> were 9, 13.5, 11, and 14 mm, respectively; those of <i>Klebsiella pneumoniae</i> were 10, 9.5, 10, and 17 mm, respectively; and those of <i>Escherichia coli</i> were 11, 11.5, 13, and 16.5 mm, respectively. ZOI results showed that CUCHNT HNMs have superior antibacterial efficacies for all tested bacterial strains compared to NT, CUNT, and CHNT. Cytotoxic capabilities of the synthesized HNMs were analyzed using the MTT assay against the HCT-116 colorectal carcinoma cell line. All HNMs showed significant anticancer activity, with CUCHNT exhibiting the most pronounced activity, having an IC<sub>50</sub> value of 87 μg/mL. Morphological changes and cell death mechanisms of synthesized HNMs were studied using dual AO/EtBr, H<sub>2</sub>DCFDA, and JC-1 staining. These findings emphasize that biosynthesized CUCHNT HNMs using <i>P. guajava</i> leaf extract could be a potential therapeutic agent for antimicrobial and anticancer applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4946-4960"},"PeriodicalIF":4.7000,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synergistic Biocidal Effects of Curcumin, Chitosan, and Nickel Titanate-Based Nanohybrids for Enhanced Antibacterial and Anticancer Therapies.\",\"authors\":\"Ebenezar Jeyasingh, Hendry Moses Panneerselvam, Karthikeyan Chandrasekaran, Sasikumar Moorthy\",\"doi\":\"10.1021/acsabm.5c00319\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Microbial infections and colorectal cancer are significant contributors to mortality and morbidity, necessitating prompt action. In the present work, NiTiO<sub>3</sub> (NT), curcumin-loaded NiTiO<sub>3</sub> (CUNT), chitosan-loaded NiTiO<sub>3</sub> (CHNT), and both curcumin and chitosan-loaded NiTiO<sub>3</sub> (CUCHNT) hybrid nanomaterials (HNMs) were systematically prepared using <i>Psidium guajava</i> leaf extract. PXRD studies revealed that NT, CUNT, CHNT, and CUCHNT exhibited a rhombohedral structure with the <i>R</i>3̅ space group with mean crystallite sizes of ∼66.41, ∼61.94, ∼61.65, and ∼65.12 nm, respectively. Band gap energies were estimated as ∼3.10, ∼3.15, ∼3.14, and ∼3.15 eV for NT, CUNT, CHNT, and CUCHNT, respectively. Antibacterial efficacies of synthesized HNMs were analyzed against both Gram-positive (G+) and Gram-negative (G-) bacteria using the well diffusion method, and the zone of inhibition (ZOI) exhibited by NT, CUNT, CHNT, and CUCHNT against <i>Staphylococcus aureus</i> were 11.5, 13, 12, and 17.5 mm, respectively; those of <i>Staphylococcus pneumoniae</i> were 9, 13.5, 11, and 14 mm, respectively; those of <i>Klebsiella pneumoniae</i> were 10, 9.5, 10, and 17 mm, respectively; and those of <i>Escherichia coli</i> were 11, 11.5, 13, and 16.5 mm, respectively. ZOI results showed that CUCHNT HNMs have superior antibacterial efficacies for all tested bacterial strains compared to NT, CUNT, and CHNT. Cytotoxic capabilities of the synthesized HNMs were analyzed using the MTT assay against the HCT-116 colorectal carcinoma cell line. All HNMs showed significant anticancer activity, with CUCHNT exhibiting the most pronounced activity, having an IC<sub>50</sub> value of 87 μg/mL. Morphological changes and cell death mechanisms of synthesized HNMs were studied using dual AO/EtBr, H<sub>2</sub>DCFDA, and JC-1 staining. These findings emphasize that biosynthesized CUCHNT HNMs using <i>P. guajava</i> leaf extract could be a potential therapeutic agent for antimicrobial and anticancer applications.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":\" \",\"pages\":\"4946-4960\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsabm.5c00319\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/14 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsabm.5c00319","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/14 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Synergistic Biocidal Effects of Curcumin, Chitosan, and Nickel Titanate-Based Nanohybrids for Enhanced Antibacterial and Anticancer Therapies.
Microbial infections and colorectal cancer are significant contributors to mortality and morbidity, necessitating prompt action. In the present work, NiTiO3 (NT), curcumin-loaded NiTiO3 (CUNT), chitosan-loaded NiTiO3 (CHNT), and both curcumin and chitosan-loaded NiTiO3 (CUCHNT) hybrid nanomaterials (HNMs) were systematically prepared using Psidium guajava leaf extract. PXRD studies revealed that NT, CUNT, CHNT, and CUCHNT exhibited a rhombohedral structure with the R3̅ space group with mean crystallite sizes of ∼66.41, ∼61.94, ∼61.65, and ∼65.12 nm, respectively. Band gap energies were estimated as ∼3.10, ∼3.15, ∼3.14, and ∼3.15 eV for NT, CUNT, CHNT, and CUCHNT, respectively. Antibacterial efficacies of synthesized HNMs were analyzed against both Gram-positive (G+) and Gram-negative (G-) bacteria using the well diffusion method, and the zone of inhibition (ZOI) exhibited by NT, CUNT, CHNT, and CUCHNT against Staphylococcus aureus were 11.5, 13, 12, and 17.5 mm, respectively; those of Staphylococcus pneumoniae were 9, 13.5, 11, and 14 mm, respectively; those of Klebsiella pneumoniae were 10, 9.5, 10, and 17 mm, respectively; and those of Escherichia coli were 11, 11.5, 13, and 16.5 mm, respectively. ZOI results showed that CUCHNT HNMs have superior antibacterial efficacies for all tested bacterial strains compared to NT, CUNT, and CHNT. Cytotoxic capabilities of the synthesized HNMs were analyzed using the MTT assay against the HCT-116 colorectal carcinoma cell line. All HNMs showed significant anticancer activity, with CUCHNT exhibiting the most pronounced activity, having an IC50 value of 87 μg/mL. Morphological changes and cell death mechanisms of synthesized HNMs were studied using dual AO/EtBr, H2DCFDA, and JC-1 staining. These findings emphasize that biosynthesized CUCHNT HNMs using P. guajava leaf extract could be a potential therapeutic agent for antimicrobial and anticancer applications.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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