{"title":"基于谱效关系分析的生炒栀子保肝活性成分研究","authors":"Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng","doi":"10.1002/bmc.70115","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.</p>\n </div>","PeriodicalId":8861,"journal":{"name":"Biomedical Chromatography","volume":"39 7","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis\",\"authors\":\"Yangang Cao, Shujing Li, Peiyang Zhang, Hongwei Li, Zhiyou Hao, Xiaoke Zheng, Weisheng Feng\",\"doi\":\"10.1002/bmc.70115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.</p>\\n </div>\",\"PeriodicalId\":8861,\"journal\":{\"name\":\"Biomedical Chromatography\",\"volume\":\"39 7\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2025-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical Chromatography\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70115\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Chromatography","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/bmc.70115","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Exploring Active Ingredients of Hepatoprotective Effect of Raw and Stir-Baked Gardeniae Fructus Based on Spectrum–Effect Relationship Analysis
Gardeniae Fructus (GF) has been used as a hepatoprotective medicine; however, the active ingredients of GF against cholestatic liver injury (CLI) remain unclear. This study aims to explore active ingredients of the hepatoprotective effects of raw GF (RGF) and stir-baked GF (SGF) by spectrum–effect relationship analysis. A total of 32 common peaks were recorded in RGF and SGF HPLC fingerprints, and nine of them were structurally characterized. Both RGF and SGF demonstrated hepatoprotective effects in an ANIT-induced CLI rat model. The spectrum–effect relationship analysis results showed that peaks 3 (gardenoside), 4, 5 (jasminoside B), 6 (genipin 1-gentiobioside), 8 (geniposide), 9, 16, 17, 19, and 22 were determined as the potential active ingredients of GF against CLI. Notably, gardenoside, jasminoside B, and genipin 1-gentiobioside exhibited good hepatoprotective effects. The research establishes a research foundation for the future quality control and medicinal application of GF.
期刊介绍：
Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.
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