Simzar Hosseinzadeh, Lida Shahghasempour, Hojjat Allah Abbaszadeh, Masoud Soleimani, Mohammad Amin Abdollahifar, Mehdi Rasouli, Mohammad Mehdi Khani, Roya Fattahi
{"title":"阿司匹林交联o -羧甲基壳聚糖水凝胶负载脂肪干细胞骨再生的体内模型","authors":"Simzar Hosseinzadeh, Lida Shahghasempour, Hojjat Allah Abbaszadeh, Masoud Soleimani, Mohammad Amin Abdollahifar, Mehdi Rasouli, Mohammad Mehdi Khani, Roya Fattahi","doi":"10.1007/s10924-025-03571-y","DOIUrl":null,"url":null,"abstract":"<div><p>Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>Optimization process of PVDF gradient unit cell lattice structures for Additive Manufacturing</p></div></div></figure></div></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 6","pages":"2931 - 2945"},"PeriodicalIF":4.7000,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vivo Model of Bone Regeneration with Aspirin Cross-linked O-carboxymethyl Chitosan Hydrogel Loaded with Adipose Derived-Stem Cells\",\"authors\":\"Simzar Hosseinzadeh, Lida Shahghasempour, Hojjat Allah Abbaszadeh, Masoud Soleimani, Mohammad Amin Abdollahifar, Mehdi Rasouli, Mohammad Mehdi Khani, Roya Fattahi\",\"doi\":\"10.1007/s10924-025-03571-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div><div><p>Optimization process of PVDF gradient unit cell lattice structures for Additive Manufacturing</p></div></div></figure></div></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":\"33 6\",\"pages\":\"2931 - 2945\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-025-03571-y\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-025-03571-y","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
In Vivo Model of Bone Regeneration with Aspirin Cross-linked O-carboxymethyl Chitosan Hydrogel Loaded with Adipose Derived-Stem Cells
Aspirin, also known as acetylsalicylic acid (ASA), a hydrophilic substance, was selected as a cross-linker to create the three-dimensional structure of the chitosan hydrogel. The main objective of this research is to incorporate aspirin as a crosslinker in hydrogels to enhance bone regeneration. O-carboxymethyl chitosan (O-CMC) was synthesized. The 0.09 O-CMC hydrogel was prepared and mixed with an aspirin solution at a concentration of 0.05 g/ml. The resulting gel composite O-CMC/ASA with and without human adipose-derived stem cell (ADSC) was then used to fill 8-mm skull defects in the Wistar model for 4 and 8 weeks, enabling an evaluation of its potential to stimulate bone formation in vivo. Histological examination and CT revealed that the hydrogel O-CMC/ASA/ADSCs promoted the migration and proliferation of ADSCs, leading to a significant increase in new bone formation compared with hydrogel O-CMC.
Graphical Abstract
Optimization process of PVDF gradient unit cell lattice structures for Additive Manufacturing
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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