基于倾斜光纤光栅强包层模式共振的夹层免疫检测传感器增强SARS-CoV-2的检测能力

IF 4.3 2区综合性期刊 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

IEEE Sensors Journal Pub Date : 2025-04-10 DOI:10.1109/JSEN.2025.3557870

Waldo Udos;Soon Hao Tan;Kok-Sing Lim;Kien Chai Ong;May Lee Low;Heming Wei;Harith Ahmad

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引用次数: 0

摘要

本研究介绍了一种利用倾斜光纤布拉格光栅（TFBG）检测严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）的方法，该方法具有强包层模式共振（~30 dB）。生物功能化处理涉及一系列表面生物功能化，包括掺入含有亲和素-生物素复合物（ABC）的增强剂层，以增强生物传感器的输出响应。研究的参数包括截止强度变化和传输光谱下包络的波长移。对于截止强度的变化，传感器的灵敏度提高~39.96%，检出限（LOD）降低~56.94%。对于波长位移，灵敏度提高~48.96%，LOD降低~64.26%。已经进行了特异性测试，包括非sars - cov -2样本的特异性测试，以展示生物传感器的强大性能。此外，还进行了对照试验，以验证生物传感器的可靠性。

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Enhanced Detectability of SARS-CoV-2 Using Sandwich Immunoassay Sensor Based on Tilted Fiber Bragg Grating With Strong Cladding Mode Resonances

This research introduces a method for detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizing tilted fiber Bragg grating (TFBG) with strong cladding mode resonances (~30 dB). The biofunctionalization treatment involves a series of surface biofunctionalizations, including the incorporation of an enhancer layer with avidin-biotin complex (ABC) to enhance the output response of the biosensor. The parameters studied include cutoff intensity change and wavelength shift of the lower envelope of the transmission spectra. For cutoff intensity change, the sensitivity of the biosensor improves by ~39.96%, and the limit of detection (LOD) decreases by ~56.94%. For wavelength shift, the sensitivity increases by ~48.96%, while the LOD is reduced by ~64.26%. Specificity tests, including those with non-SARS-CoV-2 samples, have been carried out to showcase the strong performance of the biosensor. In addition, control tests have been conducted to validate the biosensor’s reliability.

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来源期刊

IEEE Sensors Journal 工程技术-工程：电子与电气

CiteScore

7.70

自引率

14.00%

发文量

2058

审稿时长

5.2 months

期刊介绍： The fields of interest of the IEEE Sensors Journal are the theory, design , fabrication, manufacturing and applications of devices for sensing and transducing physical, chemical and biological phenomena, with emphasis on the electronics and physics aspect of sensors and integrated sensors-actuators. IEEE Sensors Journal deals with the following: -Sensor Phenomenology, Modelling, and Evaluation -Sensor Materials, Processing, and Fabrication -Chemical and Gas Sensors -Microfluidics and Biosensors -Optical Sensors -Physical Sensors: Temperature, Mechanical, Magnetic, and others -Acoustic and Ultrasonic Sensors -Sensor Packaging -Sensor Networks -Sensor Applications -Sensor Systems: Signals, Processing, and Interfaces -Actuators and Sensor Power Systems -Sensor Signal Processing for high precision and stability (amplification, filtering, linearization, modulation/demodulation) and under harsh conditions (EMC, radiation, humidity, temperature); energy consumption/harvesting -Sensor Data Processing (soft computing with sensor data, e.g., pattern recognition, machine learning, evolutionary computation; sensor data fusion, processing of wave e.g., electromagnetic and acoustic; and non-wave, e.g., chemical, gravity, particle, thermal, radiative and non-radiative sensor data, detection, estimation and classification based on sensor data) -Sensors in Industrial Practice