半乳糖基金属卟啉靶向肝癌的合成及治疗机制研究

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-15 DOI:10.1016/j.colsurfb.2025.114794

Wei Hu , Jie Zhou , Jing Huang , Yanhua Yu

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引用次数: 0

摘要

合成了靶向肝癌的四天线半乳糖金属卟啉ZnTPPGal。ZnTPPGal对HepG2细胞具有优良的荧光特性和高致死率（IC50 0.91 × 10−5 M± 2.59），在药物追踪和靶向治疗方面具有良好的潜力。相对定量蛋白质组学分析显示，ZnTPPGal诱导了475个差异表达蛋白（DEPs），其中上调174个，下调301个(Ratio >；1.5或<；0.67)。ZnTPPGal主要通过内质网应激途径和线粒体依赖途径抑制细胞存活和激活细胞凋亡，而不激活细胞自噬。EGFR、ASK1、Cat D、Rho、JNK、RagB和Rheb等功能蛋白可作为肝癌治疗和药物开发的潜在候选蛋白。

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Synthesis and study on treatment mechanism of galactosyl metalloporphyrin targeting hepatocarcinoma

Tetra-antennary galactosyl metalloporphyrin ZnTPPGal targeting hepatocarcinoma was synthesized. ZnTPPGal exhibited excellent fluorescent properties and high mortality (IC₅₀ 0.91 × 10⁻⁵ M ± 2.59) to HepG2 cells, showing good potential in drug tracing and targeted therapy. Relative quantitation proteomics analysis revealed that ZnTPPGal induced 475 differentially expressed proteins (DEPs) including 174 upregulated and 301 downregulated (Ratio > 1.5 or < 0.67). ZnTPPGal could inhibit cell survival and activate apoptosis mainly through endoplasmic reticulum stress pathway and mitochondrial dependent pathway, while did not activate autophagy. Functional proteins of EGFR, ASK1, Cat D, Rho, JNK, RagB and Rheb could be used as potential candidate proteins for hepatocarcinoma treatment and drug development.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.