评价加热温度对低温真空蒸馏植物茎体水氘和氧18偏倚的影响

IF 5.9 1区地球科学 Q1 ENGINEERING, CIVIL

Journal of Hydrology Pub Date : 2025-05-02 DOI:10.1016/j.jhydrol.2025.133408

Pei Zhao , Yan Li

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引用次数: 0

摘要

低温真空蒸馏（CVD）已被证明会严重消耗植物茎干水中的氘。本研究通过复水和锅内实验研究了不同加热温度对CVD引起的氘和氧-18偏置的影响。对于复水实验，在75°C、100°C、150°C和200°C加热温度下，样品的平均δ2H偏移量（样品同位素比值与参考值的差值）分别为- 15.4±4.3‰、- 10.8±1.1‰、- 6.6±0.9‰和- 4.5±1.0‰。与低温（75°C, 100°C）相比，高温（150°C, 200°C）显著降低了δ2H偏置。绝对含水量（AWC）对不同温度和物种的δ2H和δ18O偏移的影响显著大于相对含水量。在盆栽试验中，大部分在200°C加热温度下提取的水δ2H和δ18O与原水没有显著差异（除一种外）。然而，这些同位素偏移量与植物茎AWC呈显著负相关，表明CVD实验的最佳提取温度取决于植物含水量。更高温度下同位素偏差的改善，以及CVD实验的提取效率数据表明，在75°C和100°C下，极小部分植物水分（可能更紧密地结合在植物基质中）不能有效提取。这一小部分残留的水很可能是观测到δ2H测量偏差的主要原因。基于这些发现，建议在CVD实验中对AWC范围为1.0至1.5 g的植物进行高温提取（例如200°C），以减少植物水研究中的氘偏倚。未来的研究应该调查是否更高的加热温度可以减少已经观察到差异的实验室中的同位素偏差。

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Evaluating the effects of heating temperature on the deuterium and oxygen-18 biases of plant stems bulk water due to cryogenic vacuum distillation

Cryogenic vacuum distillation (CVD) has been proven to significantly deplete deuterium in plant stem water. In this study, rehydration and pot experiments were conducted to investigate the effects of different heating temperatures on deuterium and oxygen-18 biases caused by CVD. For rehydration experiments, the mean δ²H offsets (the difference between the isotopic ratio of a sample and the reference value) were −15.4 ± 4.3 ‰, −10.8 ± 1.1 ‰, −6.6 ± 0.9 ‰ and −4.5 ± 1.0 ‰ for 75 °C, 100 °C, 150 °C and 200 °C heating temperature, respectively. High temperatures (150 °C, 200 °C) significantly reduced δ²H bias compared to low temperatures (75 °C, 100 °C). Absolute water content (AWC) had a significantly greater influence on δ²H and δ¹⁸O offsets across temperatures and species than relative water content. In pot experiment, most water extracted at 200 °C heating temperature showed no significant difference between extracted water and source water, for both δ²H and δ¹⁸O (except one species). However, these isotopic offsets exhibited a significantly negative relationship with the AWC of plant stem, indicating that the optimal extraction temperature in CVD experiments would vary depending on the plant’s water content. The improved isotopic bias at higher temperatures, along with extraction efficiency data from CVD experiments, suggests that a very small fraction of plant water, possibly more tightly bound within the plant matrix, is not effectively extracted at 75 °C and 100 °C. This very small fraction of retained water is likely a primary contributor to the observed bias in δ²H measurements. Based on these findings, it is recommended to sample plants with AWC ranging from 1.0 to 1.5 g for high-temperature extraction (e.g., 200 °C) in CVD experiments to decrease deuterium bias for plant water studies. Future studies should investigate whether higher heating temperatures can reduce the isotopic biases in the laboratories that have observed discrepancies.

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来源期刊

Journal of Hydrology 地学-地球科学综合

CiteScore

11.00

自引率

12.50%

发文量

1309

审稿时长

7.5 months

期刊介绍： The Journal of Hydrology publishes original research papers and comprehensive reviews in all the subfields of the hydrological sciences including water based management and policy issues that impact on economics and society. These comprise, but are not limited to the physical, chemical, biogeochemical, stochastic and systems aspects of surface and groundwater hydrology, hydrometeorology and hydrogeology. Relevant topics incorporating the insights and methodologies of disciplines such as climatology, water resource systems, hydraulics, agrohydrology, geomorphology, soil science, instrumentation and remote sensing, civil and environmental engineering are included. Social science perspectives on hydrological problems such as resource and ecological economics, environmental sociology, psychology and behavioural science, management and policy analysis are also invited. Multi-and interdisciplinary analyses of hydrological problems are within scope. The science published in the Journal of Hydrology is relevant to catchment scales rather than exclusively to a local scale or site.