急性阑尾炎缺血修饰白蛋白和氧化应激标志物：一项前瞻性研究

IF 1.8 3区医学 Q2 SURGERY

Journal of Surgical Research Pub Date : 2025-05-16 DOI:10.1016/j.jss.2025.03.070

Ozan Caliskan MD , Beyza Nur Ozkan MD , Mehmet Taner Unlu MD , Nurcihan Aygun MD , Eray Metin Guler MD , Mehmet Uludag MD

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引用次数: 0

摘要

急性阑尾炎是儿童和成人常见的外科急症。然而，由于并发症的风险增加，穿孔性阑尾炎是一种需要早期诊断和治疗的疾病。在我们的研究中，我们旨在评估各种生化、血液学和炎症参数的临床价值，以区分急性阑尾炎和穿孔性阑尾炎。材料与方法本前瞻性研究共纳入了87名年龄在18-65岁之间的参与者。急性阑尾炎患者29例，穿孔性阑尾炎患者29例，健康对照29例。血清尿素、肌酐、乳酸脱氢酶和c反应蛋白浓度用自动分析仪测定，全血参数用血液学分析仪测定。用光度法测定总抗氧化状态、总氧化状态、总硫醇（TT）和天然硫醇（NT）水平。数学计算氧化应激指数和二硫化物（DIS）水平。使用市售试剂盒测定白细胞介素(IL)-1β、IL-6、肿瘤坏死因子-α和缺血修饰白蛋白（IMA）水平。结果急性阑尾炎组和穿孔阑尾炎组血清尿素、乳酸脱氢酶、c反应蛋白、白细胞、血小板计数较对照组明显升高(P <；0.001)。阑尾炎组氧化应激指标（总氧化状态、氧化应激指数、DIS、DIS/TT、DIS/NT、IMA）升高，抗氧化指标（总抗氧化状态、TT、NT、NT/TT）降低(P <；0.001)。此外，炎症标志物IL-1β、IL-6和肿瘤坏死因子-α升高，特别是在阑尾炎穿孔组(P <；0.05)。结论sima是鉴别穿孔性阑尾炎与急性阑尾炎的良好生物标志物。它的作用，结合其他氧化应激和炎症标志物，可以提高诊断的准确性，帮助临床医生及时干预，潜在地减少延迟或漏诊相关的并发症。需要进一步的研究来证实这些发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Ischemia-Modified Albumin and Oxidative Stress Marker in Acute Appendicitis: A Prospective Study

Introduction

Acute appendicitis is a common surgical emergency in childhood and adulthood. However, perforated appendicitis is a condition that requires early diagnosis and treatment due to the increased risk of complications. In our study, we aimed to evaluate the clinical values of various biochemical, hematological, and inflammatory parameters in order to distinguish acute appendicitis from perforated appendicitis.

Materials and methods

A total of 87 participants aged 18-65 y were enrolled in this prospective study. 29 patients with acute appendicitis, 29 patients with perforated appendicitis, and 29 healthy controls were included. Serum urea, creatinine, lactate dehydrogenase, and C-reactive protein concentrations were measured using an automated analyser, and whole blood parameters were measured using a haematological analyser. Total antioxidant status, total oxidant status, total thiol (TT), and native thiol (NT) levels were measured photometrically. Oxidative stress index and disulfide (DIS) level were calculated mathematically. Interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and ischemia-modified albumin (IMA) levels were measured photometrically using commercially purchased kits.

Results

The elevated levels of urea, lactate dehydrogenase, C-reactive protein, white blood cell, and platelet count in the acute and perforated appendicitis groups compared to controls, significantly (P < 0.001). Oxidative stress markers (total oxidant status, oxidative stress index, DIS, DIS/TT, DIS/NT, and IMA) were higher, while antioxidant markers (total antioxidant status, TT, NT, and NT/TT) were lower in the appendicitis groups (P < 0.001). Additionally, inflammatory markers IL-1β, IL-6, and tumor necrosis factor-α were elevated, particularly in the perforated appendicitis group (P < 0.05).

Conclusions

IMA is a promising biomarker for distinguishing perforated appendicitis from acute appendicitis. Its role, in conjunction with other oxidative stress and inflammatory markers, may enhance diagnostic precision and aid clinicians in timely intervention, potentially reducing complications associated with delayed or missed diagnoses. Further studies are warranted to validate these findings.

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来源期刊

Journal of Surgical Research 医学-外科

CiteScore

3.90

自引率

4.50%

发文量

627

审稿时长

138 days

期刊介绍： The Journal of Surgical Research: Clinical and Laboratory Investigation publishes original articles concerned with clinical and laboratory investigations relevant to surgical practice and teaching. The journal emphasizes reports of clinical investigations or fundamental research bearing directly on surgical management that will be of general interest to a broad range of surgeons and surgical researchers. The articles presented need not have been the products of surgeons or of surgical laboratories. The Journal of Surgical Research also features review articles and special articles relating to educational, research, or social issues of interest to the academic surgical community.