母代vGluT2和胚胎mGluR3信号传递系统控制着豌豆蚜虫后代翅膀的二态性

IF 4.6 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

iScience Pub Date : 2025-05-06 DOI:10.1016/j.isci.2025.112591

Yiyang Yuan , Yanyan Wang , Wanwan Ye , Liqiang Xie , Erliang Yuan , Huijuan Guo , Shifan Wang , Fang Dong , Keyan Zhu-Salzman , Feng Ge , Yucheng Sun

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引用次数: 0

摘要

蚜虫翅膀二态性是跨代表型可塑性的典型例子，但从母亲到女儿的信号传导机制尚不清楚。实验结果表明，身体接触和拥挤处理使豌豆蚜虫有翅子代比例较高。RNA测序（RNA-seq）分析结果显示，物理接触和拥挤处理均增加了脑泡性谷氨酸转运蛋白2 （ApvGluT2）和胚胎代谢性谷氨酸受体3 （ApmGluR3）的表达。在胚胎中，敲低任何一个基因都会抑制ApFoxO的磷酸化。此外，电泳迁移转移实验（EMSA）显示，在20期胚胎中，去磷酸化的ApFoxO直接结合到hedgehog基因启动子（ApHh）上，抑制其转录，导致翅状比例降低。我们的研究结果表明，大脑vGluT2和胚胎mGluR3协调传递母体身体接触信号并控制后代的翅膀发育，揭示了蚜虫身体接触依赖的跨代翅膀二态性的调节机制。

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The maternal vGluT2 and embryonic mGluR3 signaling relay system controls offspring wing dimorphism in pea aphid

Aphid wing dimorphism is a textbook example of transgenerational phenotypic plasticity, but the signaling mechanism from mother to daughter remains unclear. We showed that the physical contact and crowding treatment caused high proportion of winged offspring in the pea aphid Acyrthosiphon pisum. RNA sequencing (RNA-seq) analysis indicated that the expression of brain vesicular glutamate transporter 2 (ApvGluT2) and embryonic metabotropic glutamate receptor 3 (ApmGluR3) were increased by physical contact and crowding treatments. Knockdown of either gene inhibited phosphorylation of ApFoxO in embryos. Furthermore, electrophoretic mobility shift assays (EMSA) showed that dephosphorylated ApFoxO directly bound to the promotor of hedgehog (ApHh) to repress its transcription in stage 20 embryos, causing a lower winged proportion. Our results demonstrated that brain vGluT2 and embryonic mGluR3 coordinately relayed the maternal physical contact signals and control wing development in offspring, showcasing a regulatory mechanism underlying physical contact-dependent, transgenerational wing dimorphism in aphids.

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来源期刊

iScience Multidisciplinary-Multidisciplinary

CiteScore

7.20

自引率

1.70%

发文量

1972

审稿时长

6 weeks

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