壬醛抑制白色念珠菌生长和毒力因子

Sayali A. Chougule , Tanjila C. Gavandi , Shivani B. Patil , Sargun T. Basrani , Deepak Sawant , Shivanand R. Yankanchi , Ashwini K. Jadhav , Sankunny M. Karuppayil
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引用次数: 0

摘要

最近真菌感染的激增,特别是由白色念珠菌引起的,是一个重要的公共卫生问题,特别是在免疫功能低下的个体中。本研究旨在阐明壬醛对白色念珠菌(ATCC 90028)及其临床分离株的抗真菌活性。采用了一系列定量方法,包括最低抑制浓度(MIC)测定、细胞周期分析、生物膜测定、酵母到菌丝转变的显微镜观察、生物膜的扫描电镜观察以及麦角甾醇抑制和细胞膜损伤的评估。结果表明,壬醛分别在浓度为63 µg/ml、125 µg/ml和16 µg/ml时有效抑制白色念珠菌ATCC 90028的生长、形态发生和生物膜的形成,在浓度为125 µg/ml、500 µg/ml和63 µg/ml时有效抑制C1和C2的生长、形态发生和生物膜的形成。壬醛能诱导所有菌株的细胞周期阻滞并增加活性氧(ROS)水平。qRT-PCR分析显示,ATCC 90028和C1分离体中关键基因RAS1、BCY1、ECE1、CEK1和HWP1的表达下调,而负调控因子TUP1的表达显著上调。壬醛还影响了所有菌株麦角甾醇的合成和细胞膜。此外,壬醛在家蚕动物模型中显示出体内抗真菌作用。这些发现促进了我们对壬醛作为一种新型潜在抗真菌药物的理解,为未来的抗真菌策略奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nonanal inhibits growth and virulence factors in Candida albicans
The recent surge in fungal infections, particularly caused by Candida albicans, is an important public health concern, especially among immunocompromised individuals. The current study aims to elucidate antifungal activity of nonanal against C. albicans (ATCC 90028) and clinical isolates. A range of quantitative methods was employed, including minimum inhibitory concentration (MIC) determination, cell cycle analysis, biofilm assays, microscopic observation of yeast to hyphal transitions, scanning electron microscopy of biofilms, and assessments of ergosterol inhibition and cell membrane damage. The results demonstrated that nonanal effectively inhibits the growth, morphogenesis, and biofilm formation at concentrations of 63 µg/ml, 125 µg/ml, and 16 µg/ml, respectively for C. albicans ATCC 90028, and at 125 µg/ml, 500 µg/ml, and 63 µg/ml, respectively for C1 and C2. Nonanal was able to induce cell cycle arrest and increase the reactive oxygen species (ROS) level in all strains of C. albicans. qRT-PCR analysis showed the downregulation of expression of key genes RAS1, BCY1, ECE1, CEK1, and HWP1 in ATCC 90028 and C1 isolte, while the negative regulator TUP1 was significantly upregulated ATCC 90028. Nonanal also affected the ergosterol synthesis and cell membrane in all the tested strains. Moreover, nonanal exhibited in vivo antifungal efficacy in silkworm animal model. These findings advance our understanding of nonanal as a novel potential antifungal agent, laying the groundwork for future antifungal strategies.
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