新型硼掺杂介孔生物活性玻璃纳米颗粒负载海藻酸盐水凝胶在狗体内的评价

IF 3.2 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International dental journal Pub Date : 2025-05-15 DOI:10.1016/j.identj.2025.04.008

Marwa Samir Naga , Elbadawy Abdel Aziz Kamoun , Maha Abdel Moaty , Ahmed Zaki Ghareeb , Mona Mohy El Din , Samia Soliman Abdel Rehim Omar

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引用次数: 0

摘要

牙本质再生是保持牙齿活力的关键。本研究旨在组织学上评估一种新型可注射硼掺杂、介孔、生物活性玻璃纳米颗粒（BMBGNPs）负载海藻酸盐水凝胶的牙本质再生潜力。方法进行了新型海藻酸盐/ BMBGNPs （20 wt. %）负载复合水凝胶的配方和优化。然后将3只犬66颗牙按术后随访时间分为3组，每组22颗牙：1组2周，2组4周，3组8周。每组根据截髓填充材料进一步细分为两个亚组，其中亚组1（海藻酸盐/BMBGNPs （20 wt. %）负载水凝胶）和亚组2矿物三氧化物聚集体（MTA）。髓腔通过V类腔机械暴露。进行了完整的髓腔切开术。将测试材料放置在根状牙髓上，最后覆盖树脂复合修复体。分别于2、4、8周后处死1只。准备牙齿进行组织学评估，评估炎症细胞反应，牙髓组织组织和牙本质桥形成。采用Mann-Whitney U检验评价被试材料间组织学参数得分（P≤0.05）。结果盐水/BMBG NPs （20 wt. %）负载水凝胶后，第2周和第4周牙髓形态正常，第8周后增强（P≤0.05）。2周时出现中度炎症反应，4周、8周时好转（P≤0.05）。MTA组牙髓反应和炎症反应较差，各观察期差异有统计学意义（P≤0.05）。8周后，1组的牙本质桥最厚（P≤0.05）。结论盐酸盐/BMBG NPs （20 wt. %）负载水凝胶作为MTA的替代材料，具有牙本质再生和维持牙髓活力达到预期水平的潜力。海藻酸盐/BMBG NP负载水凝胶是重要牙髓治疗的可靠选择。

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Assessment of Novel Boron-doped Mesoporous Bioactive Glass Nanoparticles Loaded Alginate Hydrogel in Dogs

Introduction

Dentin regeneration is pivotal to preserve tooth vitality. This study aims to evaluate, histologically, the dentine regenerative potential of a novel injectable boron-doped, mesoporous, bioactive glass nanoparticle (BMBGNPs) loaded alginate hydrogel in dogs

Methods

The formulation and optimisation of the novel alginate/BMBG NPs (20 wt. %) loaded composite hydrogel were performed. Next, 66 teeth of 3 dogs were allocated into 3 groups (each including 22 teeth) according to post-operative follow-up period: group I: 2 weeks, group II: 4 weeks, and group III: 8 weeks. Each group was further subdivided according to pulpotomy filling material into two subgroups, with subgroup 1 (alginate/BMBGNPs (20 wt. %) loaded hydrogel) and subgroup 2 mineral trioxide aggregate (MTA). Pulp chambers were mechanically exposed through class V cavities. A complete pulpotomy was executed. The tested materials were positioned on the radicular pulp and finally covered with resin composite restorations. One dog was sacrificed after 2, 4, and 8 weeks. Teeth were prepared for histological evaluation assessing inflammatory cell response, pulp tissue organisation, and dentin bridge formation. The Mann-Whitney U test was employed to evaluate the scores of histological parameters between tested materials (P ≤ .05).

Results

Alginate/BMBG NPs (20 wt. %) loaded hydrogel showed normal pulp configuration at 2 and 4 weeks, which was enhanced after 8 weeks (P ≤ .05). Moderate inflammatory reaction was noted at 2 weeks, which was improved after 4 and 8 weeks (P ≤ .05). MTA group demonstrated less favourable pulpal response and inflammatory reaction with a statistically significant difference across all observational periods (P ≤ .05). After 8 weeks all teeth in group 1 exhibited the thickest dentin bridge (P ≤ .05).

Conclusions

Alginate/BMBG NPs (20 wt. %) loaded hydrogel offers the promise of regenerating dentin and maintaining pulp vitality reaching the desired level as an alternative to MTA.

Clinical Relevance

Alginate/BMBG NP loaded hydrogel is an alternative, reliable option for vital pulp therapy.

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来源期刊

International dental journal 医学-牙科与口腔外科

CiteScore

4.80

自引率

6.10%

发文量

159

审稿时长

63 days

期刊介绍： The International Dental Journal features peer-reviewed, scientific articles relevant to international oral health issues, as well as practical, informative articles aimed at clinicians.