粘弹性微流体中细胞进行性变形对mRNA的有效细胞内递送

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-05-16 DOI:10.1016/j.snb.2025.137953

Qiang Zhao, Minhui Liang, Ye Ai

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引用次数: 0

摘要

细胞内外源性生物分子的传递在生物学和医学领域是至关重要的。然而，细胞内递送的一个重大挑战是平衡细胞活力和递送效率，因为细胞大小分布广泛。在这项研究中，我们开发了一种粘弹性流体微流控装置，以实现高效的细胞内递送，并在递送后保持高细胞活力。该方法利用微收缩阵列，通过粘弹性流体在细胞膜上产生渐进剪切力，促进外源生物分子进入细胞。此外，收缩宽度大于细胞直径，以避免设备结构的物理挤压，从而保持高细胞活力并消除通道堵塞问题。在不改变收缩宽度的情况下，我们已经成功地将大范围的分子大小输送到MDA-MB-231和Jurkat细胞中。我们还证明了mRNA向Jurkat细胞的细胞内递送具有高效率（>99%），高活力（90%）和高通量（500,000个细胞/分钟）。该方法在生物科学和医学领域具有巨大的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effective intracellular delivery of mRNA by progressive cell deformation in viscoelastic microfluidics

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Effective intracellular delivery of mRNA by progressive cell deformation in viscoelastic microfluidics

Intracellular delivery of exogenous biomolecules is crucial in the fields of biology and medicine. However, a significant challenge in intracellular delivery is balancing cell viability with delivery efficiency due to the wide distribution of cell sizes. In this study, we have developed a microfluidic device with viscoelastic fluids to implement highly efficient intracellular delivery and maintain high cell viability after delivery. This method utilizes a micro-constriction array that generates progressive shear forces on the cell membrane via the viscoelastic fluids, facilitating the delivery of exogenous biomolecules into cells. Additionally, the constriction width is greater than the cell diameter to avoid physical squeezing by the device structures, which allows to maintain high cell viability and eliminate channel clogging issue. Without altering the constriction width, we have successfully delivered a wide range of molecule sizes to MDA-MB-231 and Jurkat cells. We have also demonstrated the intracellular delivery of mRNA to Jurkat cells with high efficiency (>99 %), high viability (90 %), and high throughput (500,000 cells/min). This method holds significant potential for applications in biological sciences and medicine.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.