采用微流控液滴平台设计SERS水凝胶，用于人血清中阿霉素的无标记治疗药物监测

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-05-17 DOI:10.1016/j.aca.2025.344203

Ying Wang , Jinru Cao , Ziwei Xu , Yaqin Zhan , Ziyu Xu , Hao Cheng , Wenyi Huang , Hongxing Kong , Jun Feng

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引用次数: 0

摘要

多柔比星（DOX）是一种蒽环类抗癌药物，通常用于治疗急性白血病、恶性淋巴瘤、乳腺癌和前列腺癌。由于该药物安全剂量范围窄，毒性反应强，长期或过量使用可引起严重不良反应，极大地限制了其临床应用。因此，有必要开发一种快速、敏感和可重复的DOX治疗药物监测（TDM）方法，以便根据患者的具体情况创建个性化的给药方案，从而促进疾病的精确治疗。结果以PEGDA为单体，在微流控液滴平台上制备了AgNPs包封的水凝胶微球作为SERS底物。水凝胶微球具有三维网状结构，孔径可调，有利于AgNPs在水凝胶颗粒中的均匀分散。这不仅保护了AgNPs免受生物基质的影响，增强了其稳定性和SERS信号的可重复性，而且有利于形成更密集的SERS“热点”，用于高灵敏度的SERS检测。同时，水凝胶微球的孔径可以排除生物大分子对生物基质的干扰，从而使小分子的进入具有选择性的促进作用。利用AgNPs@microgel SERS底物成功地实现了人血清中DOX的选择性、无标记SERS检测。DOX在1.0 ~ 105 ng·mL-1的浓度范围内线性良好，检出限为1.0 ng·mL-1。该方法具有快速、灵敏、重现性好、不需要样品前处理、样品消耗量小等特点。为高灵敏度、可重复的无标记检测人血清中DOX提供了一条新的途径。同时，新方法具有检测更多小分子药物的潜力，在临床TDM中具有广阔的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

SERS hydrogel designed using a microfluidic droplet platform for label-free therapeutic drug monitoring of doxorubicin in human serum

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SERS hydrogel designed using a microfluidic droplet platform for label-free therapeutic drug monitoring of doxorubicin in human serum

Background

Doxorubicin (DOX) is an anthracycline anticancer drug that is commonly employed in the treatment of acute leukemia, malignant lymphoma, breast cancer and prostate cancer. Given the narrow safe dosage range and the strong toxic reaction associated with the drug, prolonged or excessive use can result in serious adverse reactions, which greatly limits its clinical application. It is therefore necessary to develop a rapid, sensitive and reproducible therapeutic drug monitoring (TDM) method for DOX in order to create an individualised dosing regimen based on the patient's specific situation, thus facilitating the precise treatment of the disease.

Results

We prepared hydrogel microspheres encapsulated with AgNPs as SERS substrates on a microfluidic droplet platform, utilising PEGDA as the monomer. The hydrogel microspheres have a 3D mesh structure with adjustable pore size, which facilitates the uniform dispersion of AgNPs in the hydrogel particles. This not only safeguards AgNPs from the biological matrix and enhances their stability and SERS signal reproducibility, but also facilitates the formation of denser SERS "hotspots" for highly sensitive SERS detection. Concurrently, the pore size of the hydrogel microspheres can preclude the interference of biomacromolecules in the biological matrix, thereby enabling the selective facilitation of the entry of small molecules. The selective, label-free SERS detection of DOX within human serum was successfully achieved by employing AgNPs@microgel SERS substrates. The linearity of DOX was demonstrated within the concentration range of 1.0–10⁵ ng mL⁻¹, with a detection limit of 1.0 ng mL⁻¹.

Significance

The method is characterised by its rapidity, sensitivity, reproducibility, the absence of any sample pretreatment requirements and minimal sample consumption. As a consequence, it provides a new avenue for the highly sensitive and reproducible label-free detection of DOX in human serum. Meanwhile, the new method has the potential to detect more small molecule drugs, which has a broad application potential in clinical TDM.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.