全基因组高通量转座子突变揭示了白喉棒状杆菌适应酸性pH的关键因素。

IF 2.6 4区 生物学 Q3 MICROBIOLOGY
Camila Azevedo Antunes, Emily C A Goodall, Ian R Henderson, David Wild, Alexander Mehltretter, Philipp Ott, Markus Hölzl, Lisa Ott, Gerald Seidel, Andreas Burkovski
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引用次数: 0

摘要

白喉棒状杆菌是一种引起危及生命的白喉疾病的著名病原体,在宿主内遇到恶劣的细胞内环境,特别是在酸性条件占优势的巨噬细胞内。为了阐明其酸性胁迫反应的遗传和分子机制,我们采用了转座子定向插入位点测序方法。这项综合研究确定了促进白喉白喉在低pH条件下存活的关键基因和途径。在随后的实验中,Ktr钾转运系统被认为是在酸性胁迫下维持pH稳态和生长的关键因素。ktrBA缺失菌株在pH为5时生长明显下降,通过反式表达ktrBA可以恢复生长。与野生型相比,缺失菌株在巨噬细胞中的摄取和存活没有变化,这表明Ktr系统对白喉白喉在吞噬细胞中的存活并不重要。这些发现促进了我们对白喉芽孢杆菌病理生理学的理解,进一步描绘了白喉芽孢杆菌在恶劣环境中的复杂生存策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome-wide high-throughput transposon mutagenesis unveils key factors for acidic pH adaptation of Corynebacterium diphtheriae.

Corynebacterium diphtheriae, a notable pathogen responsible for the life-threatening disease diphtheria, encounters harsh intracellular environments within the host, particularly within macrophages where acidic conditions prevail. To elucidate the genetic and molecular mechanisms underlying its acid stress response, we employed a Transposon Directed Insertion-site Sequencing approach. This comprehensive study identified crucial genes and pathways facilitating C. diphtheriae's survival at low pH. In subsequent experiments, the Ktr potassium transport system was identified as a putative key factor for maintaining pH homeostasis and growth under acidic stress. A ktrBA deletion strain exhibited significantly reduced growth at pH 5, which could be restored by ktrBA expression in trans. The deletion strain showed unchanged uptake and survival in macrophages compared to the wild-type, indicating that the Ktr system is not crucial for the survival of C. diphtheriae in phagocytes. These findings advance our understanding of C. diphtheriae's pathophysiology, further delineating the intricate survival strategies of C. diphtheriae in hostile environments.

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来源期刊
Microbiology-Sgm
Microbiology-Sgm 生物-微生物学
CiteScore
4.60
自引率
7.10%
发文量
132
审稿时长
3.0 months
期刊介绍: We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.
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