异鼠李素的机制:靶向MAPK和NF-κB通路减轻脂多糖诱导的炎症。

Abdelrahim Alqudah, Muna Barakat, Lujain F Alzaghari, Esam Qnais, Omar Gammoh, Mohammad Alqudah, Alaa Aa Aljabali, Sireen Abdul Rahim Shilbayeh
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引用次数: 0

摘要

慢性炎症可导致粘膜损伤,表现为疼痛、水肿、抽搐和发热症状。本研究探讨了异鼠李素(ISO)的抗炎特性及其作为药物的潜力。方法采用脂多糖(LPS)激活巨噬细胞的体外实验,评价ISO对炎症的影响。我们专注于量化lps刺激的RAW 264.7细胞中促炎细胞因子,白细胞介素[IL]-1β, IL-6和肿瘤坏死因子[TNF-α]以及介质,如一氧化氮[NO]和前列腺素E2 [PGE2]的合成。结果ISO在保持细胞完整性的同时显著降低NO和PGE2水平。ISO以剂量依赖的方式减少了促炎细胞因子的合成。此外,ISO处理降低了诱导型一氧化氮合酶(iNOS)和环氧合酶-2 (COX-2)的mRNA水平,LPS处理后这两种酶的mRNA水平升高。机制研究表明,ISO的抗炎特性是通过抑制丝裂原活化蛋白激酶[MAPK]家族磷酸化和下调核因子-κB抑制剂[IκB-α]在MAPK和核因子-κB [NF-κB]通路中发挥作用的。结论本研究结果表明,ISO可通过特异性抑制炎性途径治疗炎性疾病。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanistic Insights into Isorhamnetin: Targeting MAPK and NF-κB Pathways to Mitigate LPS-Induced Inflammation.

Introduction Chronic inflammation may result in mucosal damage, presenting as pain, edema, convulsions, and fever symptoms. This study investigated the anti-inflammatory characteristics of isorhamnetin (ISO) and its potential as a medicinal agent. Method In this study, in vitro tests were performed in which macrophages were activated with lipopolysaccharide (LPS) to evaluate the effect of ISO on inflammation. We concentrated on quantifying the synthesis of pro-inflammatory cytokines, interleukin [IL]-1β, IL-6, and tumor necrosis factor [TNF-α], as well as mediators, such as nitric oxide [NO] and prostaglandin E2 [PGE2], in LPS-stimulated RAW 264.7 cells. Results The findings indicated that ISO significantly decreased levels of NO and PGE2 while maintaining cellular integrity. ISO reduced the synthesis of pro-inflammatory cytokines in a dose-dependent manner. Moreover, ISO treatment decreased mRNA levels of inducible nitric oxide synthase [iNOS] and cyclooxygenase-2 [COX-2], which were enhanced following LPS exposure. Mechanistic investigations revealed that the antiinflammatory properties of ISO were facilitated by the inhibition of phosphorylation in the mitogen-activated protein kinase [MAPK] family and the downregulation of nuclear factor-kappa B inhibitor [IκB-α] within both the MAPK and nuclear factor-kappa B [NF-κB] pathways. Conclusion These findings establish ISO as a viable alternative for treating inflammatory diseases by specifically inhibiting essential inflammatory pathways.

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