Thomas Kozusnik, Carole Kebbi-Beghdadi, Silvia Ardissone, Simone E Adams, Gilbert Greub
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Moreover, among all the stressors examined, only heat shock induced a strong upregulation of <i>wcw_0502</i> and its <i>C. trachomatis</i> homologue, <i>ctl0271</i>. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of <i>wcw_0502</i> and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the <i>wcw_0502</i> promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to <i>wcw_0502</i> upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.</p>","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"171 4","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038028/pdf/","citationCount":"0","resultStr":"{\"title\":\"A conserved <i>Chlamydiota</i>-specific Type III Secretion System effector linked to stress response.\",\"authors\":\"Thomas Kozusnik, Carole Kebbi-Beghdadi, Silvia Ardissone, Simone E Adams, Gilbert Greub\",\"doi\":\"10.1099/mic.0.001545\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Despite broad genetic variability, members of the <i>Chlamydiota</i> phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In <i>Waddlia chondrophila</i>, <i>wcw_0502</i> RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the <i>wcw_0502</i> gene expression upon iron starvation is not conserved in other chlamydial species such as <i>Chlamydia trachomatis</i>, <i>Chlamydia pneumoniae</i>, <i>Simkania negevensis</i> or <i>Estrella lausannensis</i>. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of <i>wcw_0502</i> and its <i>C. trachomatis</i> homologue, <i>ctl0271</i>. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of <i>wcw_0502</i> and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the <i>wcw_0502</i> promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to <i>wcw_0502</i> upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. 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A conserved Chlamydiota-specific Type III Secretion System effector linked to stress response.
Despite broad genetic variability, members of the Chlamydiota phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In Waddlia chondrophila, wcw_0502 RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the wcw_0502 gene expression upon iron starvation is not conserved in other chlamydial species such as Chlamydia trachomatis, Chlamydia pneumoniae, Simkania negevensis or Estrella lausannensis. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of wcw_0502 and its C. trachomatis homologue, ctl0271. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of wcw_0502 and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the wcw_0502 promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to wcw_0502 upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.
期刊介绍:
We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms.
Topics include but are not limited to:
Antimicrobials and antimicrobial resistance
Bacteriology and parasitology
Biochemistry and biophysics
Biofilms and biological systems
Biotechnology and bioremediation
Cell biology and signalling
Chemical biology
Cross-disciplinary work
Ecology and environmental microbiology
Food microbiology
Genetics
Host–microbe interactions
Microbial methods and techniques
Microscopy and imaging
Omics, including genomics, proteomics and metabolomics
Physiology and metabolism
Systems biology and synthetic biology
The microbiome.