一个保守的衣原体特异性III型分泌系统效应与应激反应相关。

IF 2.6 4区 生物学 Q3 MICROBIOLOGY
Thomas Kozusnik, Carole Kebbi-Beghdadi, Silvia Ardissone, Simone E Adams, Gilbert Greub
{"title":"一个保守的衣原体特异性III型分泌系统效应与应激反应相关。","authors":"Thomas Kozusnik, Carole Kebbi-Beghdadi, Silvia Ardissone, Simone E Adams, Gilbert Greub","doi":"10.1099/mic.0.001545","DOIUrl":null,"url":null,"abstract":"<p><p>Despite broad genetic variability, members of the <i>Chlamydiota</i> phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In <i>Waddlia chondrophila</i>, <i>wcw_0502</i> RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the <i>wcw_0502</i> gene expression upon iron starvation is not conserved in other chlamydial species such as <i>Chlamydia trachomatis</i>, <i>Chlamydia pneumoniae</i>, <i>Simkania negevensis</i> or <i>Estrella lausannensis</i>. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of <i>wcw_0502</i> and its <i>C. trachomatis</i> homologue, <i>ctl0271</i>. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of <i>wcw_0502</i> and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the <i>wcw_0502</i> promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to <i>wcw_0502</i> upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.</p>","PeriodicalId":49819,"journal":{"name":"Microbiology-Sgm","volume":"171 4","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038028/pdf/","citationCount":"0","resultStr":"{\"title\":\"A conserved <i>Chlamydiota</i>-specific Type III Secretion System effector linked to stress response.\",\"authors\":\"Thomas Kozusnik, Carole Kebbi-Beghdadi, Silvia Ardissone, Simone E Adams, Gilbert Greub\",\"doi\":\"10.1099/mic.0.001545\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Despite broad genetic variability, members of the <i>Chlamydiota</i> phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In <i>Waddlia chondrophila</i>, <i>wcw_0502</i> RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the <i>wcw_0502</i> gene expression upon iron starvation is not conserved in other chlamydial species such as <i>Chlamydia trachomatis</i>, <i>Chlamydia pneumoniae</i>, <i>Simkania negevensis</i> or <i>Estrella lausannensis</i>. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of <i>wcw_0502</i> and its <i>C. trachomatis</i> homologue, <i>ctl0271</i>. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of <i>wcw_0502</i> and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the <i>wcw_0502</i> promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to <i>wcw_0502</i> upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.</p>\",\"PeriodicalId\":49819,\"journal\":{\"name\":\"Microbiology-Sgm\",\"volume\":\"171 4\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038028/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbiology-Sgm\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/mic.0.001545\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbiology-Sgm","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/mic.0.001545","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

尽管有广泛的遗传变异,衣原体门的成员共享一个关键的应激反应表型,形成异常体。然而,在暴露于不同种类的压力源时,这种反应是如何运作的,在很大程度上仍然是未知的。在Waddlia软骨细胞中,wcw_0502 RNA水平在铁饥饿诱导的异常体中上调。Wcw_0502是一种推测的III型分泌系统(T3SS)效应物,在所有已知的衣原体物种中都有同源物,无论其宿主如何。然而,铁饥饿时wcw_0502基因表达的上调在其他衣原体物种如沙眼衣原体、肺炎衣原体、阴性Simkania negevensis或洛桑Estrella lausannensis中并不保守。此外,在所有被检测的应激源中,只有热休克导致wcw_0502及其沙眼衣原体同源物ctl0271的强烈上调。在wcw_0502及其同源物的启动子区存在一个控制伴侣表达序列的反向重复。我们假设,在没有压力的情况下,保守的抑制因子HrcA与Hsp60伴侣结合,抑制转录。在铁饥饿诱导的异常体中,与网状体相比,HrcA和Hsp60在wcw_0502启动子区域的占用减少,这可能导致wcw_0502上调。这种新描述的T3SS效应的确切功能尚不清楚。在Wcw_0502的c端区域发现了胱氨酸结样结构域,这是细菌蛋白中从未描述过的结构特征。这种结构被描述为对蛋白质水解、化学和热应激源具有高度抗性,这是在影响蛋白质完整性的应激过程中分泌蛋白质产量增加的有利特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A conserved Chlamydiota-specific Type III Secretion System effector linked to stress response.

Despite broad genetic variability, members of the Chlamydiota phylum share a crucial stress response phenotype, the formation of aberrant bodies. However, how this response operates upon exposure to different kinds of stressors is still largely unknown. In Waddlia chondrophila, wcw_0502 RNA levels are upregulated in aberrant bodies induced by iron starvation. Wcw_0502 is a putative type III secretion system (T3SS) effector and has a homologue in every known chlamydial species, regardless of their host. However, the upregulation of the wcw_0502 gene expression upon iron starvation is not conserved in other chlamydial species such as Chlamydia trachomatis, Chlamydia pneumoniae, Simkania negevensis or Estrella lausannensis. Moreover, among all the stressors examined, only heat shock induced a strong upregulation of wcw_0502 and its C. trachomatis homologue, ctl0271. A Controlling Inverted Repeat of Chaperone Expression sequence is present in the promoter region of wcw_0502 and its homologues. We hypothesized that in the absence of stress, the conserved repressor HrcA, in association with the Hsp60 chaperone, binds this sequence and represses transcription. A decreased occupancy of HrcA and Hsp60 at the wcw_0502 promoter region was observed in aberrant bodies induced by iron starvation when compared to reticulate bodies, which may lead to wcw_0502 upregulation. The precise function of this newly described T3SS effector is still unclear. A cystine knot-like domain, a structural feature never described before in bacterial proteins, was found in the C-terminal region of Wcw_0502. This structure is described as highly resistant to proteolytic, chemical and thermic stressors, an advantageous property for a secreted protein with an increased production during stresses that impact protein integrity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Microbiology-Sgm
Microbiology-Sgm 生物-微生物学
CiteScore
4.60
自引率
7.10%
发文量
132
审稿时长
3.0 months
期刊介绍: We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信