超高效液相色谱-串联质谱法测定去氧胆酸及其在人血浆药代动力学研究中的应用。

IF 2.5 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

SLAS Technology Pub Date : 2025-04-10 DOI:10.1016/j.slast.2025.100289

Wanlin Xi, Diyi Fu, Ni Wu, Fei Liu, Cuixia Zhang, Ruijie Wan, Zhen Wu, Rui Chen, Qian Zhao

引用次数: 0

摘要

脱氧胆酸（DCA）注射用于治疗中度至重度的颏下隆起或面部丰满引起的过多的颏下脂肪堆积。利用超高效液相色谱-串联质谱（UPLC-MS/MS）快速、准确、可靠地测定了人血浆中的DCA，定量限低至56 ng/mL。选取6个健康个体低浓度内源性去氧胆酸空白血浆，混合制备标准曲线样品。材料通过蛋白水解纯化，随后使用BEH C18色谱柱（2.1 × 50 mm, 1.7µm）进行分离。实验采用多反应监测（MRM）和ESI源在负模式下工作，对质量进行了鉴定和测量。DCA和DCA-d4（同位素内标）分别在m/z 391.2- → 345.2-和m/z 395.2- → 349.2-处观察到离子电荷转移的前体。对该方法进行了综合评价，包括线性、灵敏度、重现性、选择性、鲁棒性、矩阵效应、准确性、携带性和回收率。总之，验证结果表明，该模型具有较高的可靠性、准确性和精密度，可以有效地用于在随机、平行、对照的I期临床试验中研究DCA的药代动力学特性。

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WITHDRAWN: An ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of deoxycholic acid and application to a pharmacokinetic study in human plasma.

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来源期刊

SLAS Technology Computer Science-Computer Science Applications

CiteScore

6.30

自引率

7.40%

发文量

审稿时长

106 days

期刊介绍： SLAS Technology emphasizes scientific and technical advances that enable and improve life sciences research and development; drug-delivery; diagnostics; biomedical and molecular imaging; and personalized and precision medicine. This includes high-throughput and other laboratory automation technologies; micro/nanotechnologies; analytical, separation and quantitative techniques; synthetic chemistry and biology; informatics (data analysis, statistics, bio, genomic and chemoinformatics); and more.