c-di-GMP效应物FleQ通过抑制黄氏固氮菌中algD的转录来控制海藻酸盐的产生。

IF 2.6 4区 生物学 Q3 MICROBIOLOGY
Víctor V Barrios-Rafael, Carlos L Ahumada-Manuel, Scherezada Orgaz-Ramírez, Jessica Nava-Galeana, Josefina Guzmán, Soledad Moreno, Víctor H Bustamante, Cinthia Núñez
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引用次数: 0

摘要

作为假单胞菌科的一员,Azotobacter vinelandii的胞外多糖海藻酸盐的生产受到第二信使c-di-GMP的积极控制。这种作用完全归因于c-二- gmp在激活海藻酸盐聚合酶复合体中的作用。本研究探讨了c-di-GMP在海藻酸盐合成关键酶algD转录中的作用。algD转录与人为的高或低c-二- gmp水平相关。此外,研究发现,作为最具特征的c-di-GMP效应物之一,FleQ对海藻酸盐的产生和algD的转录产生负面影响,因为与野生型菌株或ΔfleQ/ FleQ +互补菌株相比,ΔfleQ突变体中这两个因素都增加了。电泳迁移率转移试验(EMSAs)证实FleQ直接结合到algD的调控区域,这与在algD rpos依赖启动子附近存在两个FleQ结合位点是一致的。在A. vinelandii中,c-di-GMP对于海藻酸C-5外膜酶(AlgE1-6)的表达至关重要,alg酸C-5外膜酶是构建分化细胞包膜所必需的。然而,FleQ并没有参与这项规定。总之,我们的研究结果支持了一个模型,在这个模型中,algD转录受到c-di-GMP的阳性控制,而FleQ可能只是部分地介导了这种作用。相比之下,我们的研究揭示了一个不依赖于fleq的调控机制来控制葡萄球菌的囊化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The c-di-GMP effector FleQ controls alginate production by repressing transcription of algD in Azotobacter vinelandii.

Production of the exopolysaccharide alginate by Azotobacter vinelandii, member of the Pseudomonadaceae family, is positively controlled by the second messenger c-di-GMP. This effect was solely attributed to the role of c-di-GMP in activating the alginate polymerase complex. In this study, the role of c-di-GMP in algD transcription, which encodes the key enzyme for alginate synthesis, was investigated. algD transcription correlated with artificially high or low levels of c-di-GMP. Moreover, FleQ, one of the best-characterized c-di-GMP effectors, was found to exert a negative effect on alginate production and algD transcription, as both increased in a ΔfleQ mutant relative to the wild-type strain or the ΔfleQ/fleQ+ complemented strain. Electrophoretic mobility shift assays (EMSAs) confirmed that FleQ directly binds to the regulatory region of algD, which was consistent with the presence of two FleQ binding sites in the vicinity of the algD RpoS-dependent promoter. In A. vinelandii, c-di-GMP is essential for the expression of alginate C-5 epimerases (AlgE1-6), which are necessary for structuring the envelope of differentiated cells, known as cysts. However, FleQ was not involved in this regulation. Collectively, our results support a model in which algD transcription is under the positive control of c-di-GMP, while FleQ may only partially mediate this effect. In contrast, our study revealed a FleQ-independent regulatory mechanism for the control of A. vinelandii encystment.

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来源期刊
Microbiology-Sgm
Microbiology-Sgm 生物-微生物学
CiteScore
4.60
自引率
7.10%
发文量
132
审稿时长
3.0 months
期刊介绍: We publish high-quality original research on bacteria, fungi, protists, archaea, algae, parasites and other microscopic life forms. Topics include but are not limited to: Antimicrobials and antimicrobial resistance Bacteriology and parasitology Biochemistry and biophysics Biofilms and biological systems Biotechnology and bioremediation Cell biology and signalling Chemical biology Cross-disciplinary work Ecology and environmental microbiology Food microbiology Genetics Host–microbe interactions Microbial methods and techniques Microscopy and imaging Omics, including genomics, proteomics and metabolomics Physiology and metabolism Systems biology and synthetic biology The microbiome.
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