{"title":"胶体金免疫层析试纸条用于临床样品中犬尿氨酸的检测。","authors":"Jingqi Dong, Jialin Hu, Liqiang Liu, Aihua Qu, Hua Kuang and Chuanlai Xu","doi":"10.1039/D5TB00188A","DOIUrl":null,"url":null,"abstract":"<p >Kynurenine (KYN), a metabolite of tryptophan, is currently receiving increasing attention as one of the biomarkers for clinical disease monitoring. To realize the determination of KYN in human plasma samples in the clinic, a specific monoclonal antibody (mAb) against KYN was prepared based on the structural characteristics of KYN. By optimizing the detection conditions, we obtained anti-KYN mAb 1G10 with a sensitivity of 143.66 ng mL<small><sup>−1</sup></small>, and established a colloidal gold immunochromatographic assay (CGCA) platform for the qualitative and quantitative analysis of KYN in human plasma samples, using CGCA strips to meet the needs of clinical testing. The recoveries of the strips for the determination of KYN in plasma samples were in the range of 85.22–99.72%, which were validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the results were in good agreement with the LC-MS/MS method, indicating that the accuracy of the prepared CGCA strips was high. Based on the characteristics of low cost, high throughput and good specificity, the test strips will be widely applicable for clinical determination.</p>","PeriodicalId":83,"journal":{"name":"Journal of Materials Chemistry B","volume":" 20","pages":" 5911-5918"},"PeriodicalIF":6.1000,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Colloidal gold immunochromatographic strips for the detection of kynurenine in clinical samples†\",\"authors\":\"Jingqi Dong, Jialin Hu, Liqiang Liu, Aihua Qu, Hua Kuang and Chuanlai Xu\",\"doi\":\"10.1039/D5TB00188A\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Kynurenine (KYN), a metabolite of tryptophan, is currently receiving increasing attention as one of the biomarkers for clinical disease monitoring. To realize the determination of KYN in human plasma samples in the clinic, a specific monoclonal antibody (mAb) against KYN was prepared based on the structural characteristics of KYN. By optimizing the detection conditions, we obtained anti-KYN mAb 1G10 with a sensitivity of 143.66 ng mL<small><sup>−1</sup></small>, and established a colloidal gold immunochromatographic assay (CGCA) platform for the qualitative and quantitative analysis of KYN in human plasma samples, using CGCA strips to meet the needs of clinical testing. The recoveries of the strips for the determination of KYN in plasma samples were in the range of 85.22–99.72%, which were validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the results were in good agreement with the LC-MS/MS method, indicating that the accuracy of the prepared CGCA strips was high. Based on the characteristics of low cost, high throughput and good specificity, the test strips will be widely applicable for clinical determination.</p>\",\"PeriodicalId\":83,\"journal\":{\"name\":\"Journal of Materials Chemistry B\",\"volume\":\" 20\",\"pages\":\" 5911-5918\"},\"PeriodicalIF\":6.1000,\"publicationDate\":\"2025-04-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Materials Chemistry B\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/tb/d5tb00188a\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Materials Chemistry B","FirstCategoryId":"1","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/tb/d5tb00188a","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
犬尿氨酸（KYN）是色氨酸的代谢物，目前作为临床疾病监测的生物标志物之一受到越来越多的关注。为了实现临床对人血浆样品中KYN的检测，根据KYN的结构特点制备了针对KYN的特异性单克隆抗体（mAb）。通过优化检测条件，获得灵敏度为143.66 ng mL-1的抗KYN单抗1G10，并建立了用于人血浆样品中KYN定性、定量分析的胶体金免疫层析分析（CGCA）平台，使用CGCA试纸条满足临床检测需要。经液相色谱-串联质谱（LC-MS/MS）验证，该条带测定血浆样品中KYN的回收率为85.22 ~ 99.72%，与LC-MS/MS方法吻合较好，说明所制备的CGCA条带具有较高的准确度。该试纸条具有成本低、通量高、特异性好的特点，将广泛应用于临床检测。
Colloidal gold immunochromatographic strips for the detection of kynurenine in clinical samples†
Kynurenine (KYN), a metabolite of tryptophan, is currently receiving increasing attention as one of the biomarkers for clinical disease monitoring. To realize the determination of KYN in human plasma samples in the clinic, a specific monoclonal antibody (mAb) against KYN was prepared based on the structural characteristics of KYN. By optimizing the detection conditions, we obtained anti-KYN mAb 1G10 with a sensitivity of 143.66 ng mL−1, and established a colloidal gold immunochromatographic assay (CGCA) platform for the qualitative and quantitative analysis of KYN in human plasma samples, using CGCA strips to meet the needs of clinical testing. The recoveries of the strips for the determination of KYN in plasma samples were in the range of 85.22–99.72%, which were validated by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and the results were in good agreement with the LC-MS/MS method, indicating that the accuracy of the prepared CGCA strips was high. Based on the characteristics of low cost, high throughput and good specificity, the test strips will be widely applicable for clinical determination.
期刊介绍：
Journal of Materials Chemistry A, B & C cover high quality studies across all fields of materials chemistry. The journals focus on those theoretical or experimental studies that report new understanding, applications, properties and synthesis of materials. Journal of Materials Chemistry A, B & C are separated by the intended application of the material studied. Broadly, applications in energy and sustainability are of interest to Journal of Materials Chemistry A, applications in biology and medicine are of interest to Journal of Materials Chemistry B, and applications in optical, magnetic and electronic devices are of interest to Journal of Materials Chemistry C.Journal of Materials Chemistry B is a Transformative Journal and Plan S compliant. Example topic areas within the scope of Journal of Materials Chemistry B are listed below. This list is neither exhaustive nor exclusive：
Antifouling coatings
Biocompatible materials
Bioelectronics
Bioimaging
Biomimetics
Biomineralisation
Bionics
Biosensors
Diagnostics
Drug delivery
Gene delivery
Immunobiology
Nanomedicine
Regenerative medicine & Tissue engineering
Scaffolds
Soft robotics
Stem cells
Therapeutic devices
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