SNHG19通过调控miR-137/蛋白酪氨酸磷酸酶4A3轴促进肝癌的增殖转移。

IF 2.3 Q2 MEDICINE, GENERAL & INTERNAL
SAGE Open Medicine Pub Date : 2025-04-21 eCollection Date: 2025-01-01 DOI:10.1177/20503121251334272
Yuting Ding, Wenkang Luan, Feng Lu, Zhe Wang, Xuanlin Shen
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引用次数: 0

摘要

目的:长链非编码rna在肿瘤生物学中起着重要作用。SNHG19在一些恶性肿瘤中被发现是一个新的致癌lncRNA。然而,SNHG19在肝细胞癌中的作用尚未见报道。方法:采用实时荧光定量逆转录聚合酶链式反应(qRT-PCR)检测SNHG19在肝癌组织中的表达。来自癌症基因组图谱的黑色素瘤病例被纳入本研究。采用细胞计数试剂盒-8法、Transwell法和抓伤法探讨SNHG19在黑色素瘤细胞中的作用。采用荧光素酶报告基因法和RNA下拉法探讨SNHG19在肝细胞癌中的分子机制。结果:我们发现SNHG19在肝细胞癌中表达上调。高水平SNHG19的肝细胞癌患者无病生存期(RFS)较短。SNHG19通过海绵吸附miR-137释放蛋白酪氨酸磷酸酶4A3 mRNA转录物来促进蛋白酪氨酸磷酸酶4A3的表达。SNHG19通过影响miR-137/蛋白酪氨酸磷酸酶4A3轴促进肝细胞癌的发展。结论:SNHG19在肝细胞癌发生发展中的作用。SNHG19可能作为肝细胞癌患者的特异性分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SNHG19 promotes the proliferation and metastasis of hepatocellular carcinoma through regulating the miR-137/protein tyrosine phosphatase 4A3 axis.

Objectives: Long noncoding RNAs plays the important part in tumor biology. SNHG19 was found to be a new oncogenic lncRNA in some malignant tumors. However, the effect of SNHG19 in hepatocellular carcinoma has not been reported.

Methods: The expression of SNHG19 in hepatocellular carcinoma tissues were detected by using quantitative Real-Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Melanoma cases from The Cancer Genome Atlas were included in this study. cell counting kit-8 assay, Transwell, and scratch wound assay were used to explore the role of SNHG19 in melanoma cells. Luciferase reporter assays and RNA pull-down assay were used to explore the molecular mechanism of SNHG19 in hepatocellular carcinoma.

Results: Here, we found that SNHG19 level was upregulated in hepatocellular carcinoma. Hepatocellular carcinoma patients with high levels of SNHG19 have shorter Disease-Free Survival (RFS). SNHG19 promotes the Protein Tyrosine Phosphatase 4A3 expression by sponging miR-137 to liberate Protein Tyrosine Phosphatase 4A3 mRNA transcripts. SNHG19 enhances the development of hepatocellular carcinoma by affecting miR-137/Protein Tyrosine Phosphatase 4A3 axis.

Conclusion: These results demonstrated the effect of SNHG19 in the occurrence and progression of hepatocellular carcinoma. SNHG19 may be used as the specific molecular target in patients with hepatocellular carcinoma.

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来源期刊
SAGE Open Medicine
SAGE Open Medicine MEDICINE, GENERAL & INTERNAL-
CiteScore
3.50
自引率
4.30%
发文量
289
审稿时长
12 weeks
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