恢复期血清对三种不同SARS-CoV-2毒株的总抗体和中和能力。

Q3 Medicine
N V Palyanova, L S Adamenko, O G Kurskaya, T A Saroyan, M V Solomatina, I A Sobolev, A M Shestopalov
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引用次数: 0

摘要

该研究的目的是评估COVID-19康复者对SARS-CoV-2的体液免疫水平。材料和方法:采用ELISA进行抗体定量,利用3株SARS-CoV-2病毒进行微量中和试验测定中和活性,利用Illumina MiSeq平台进行NGS测序,利用NextClade资源进行系统发育分析。结果:康复者抗体平均浓度为133.42±7.2 BAU/mL,与患者性别、年龄无关,与感染时间无关。对研究的SARS-CoV-2菌株进行测序并存入国际GISAID数据库。根据遗传分析:EPI_ISL_19424272在系统发育上属于B1.1分支,EPI_ISL_19424271 -属于B.1.1.397分支,EPI_ISL_19424270 -属于Delta B.1.617.2.122。恢复期血清(在研究前2-3个月患病的人)对前两种SARS-CoV-2变体的中和能力没有显著差异,而对后来出现在新西伯利亚地区的Delta变体的中和能力显著降低。结论:恢复期血清对已康复的病毒变体的中和活性最高,而对新变体的中和活性降低或缺失。针对SARS-CoV-2原始变体开发的抗体可能对新出现的毒株不够有效,因为病毒中出现了突变,使其能够逃避先前形成的体液免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Total antibodies and neutralizing ability of convalescent sera against three different strains of SARS-CoV-2.

The aim of the study was to assess the level of humoral immunity to SARS-CoV-2 in COVID-19 convalescents.

Materials and methods: We used ELISA for antibody quantitation, microneutralization test using three SARS-CoV-2 strains for neutralizing activity measurement, Illumina MiSeq platform for NGS sequencing and NextClade resource for phylogenetic analysis.

Results: The mean concentration of antibody in convalescents was 133.42 ± 7.2 BAU/mL and the value depended on neither the gender and age of the patients, nor on the time elapsed since COVID-19 infection. The studied SARS-CoV-2 strains were sequenced and deposited in the international GISAID database. According to genetic analysis: EPI_ISL_19424272 phylogenetically belongs to the B1.1 clade, EPI_ISL_19424271 - to B.1.1.397 clade, EPI_ISL_19424270 - to Delta B.1.617.2.122. There were no significant differences in the neutralizing ability of convalescent sera (those who had been ill 2-3 months before the study) for the first two variants of SARS-CoV-2 and it was significantly reduced for the Delta variant, which appeared in the Novosibirsk region later.

Conclusions: The neutralizing activity of convalescent sera was the highest against those variants of the virus that the patient had recovered from, while was reduced or absent against the new variant. The antibody developed to the original variants of the SARS-CoV-2 may not be effective enough against newly emerging strains due to the emergence of mutations in the virus that allow it to evade previously developed humoral immune response.

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来源期刊
Voprosy virusologii
Voprosy virusologii Medicine-Infectious Diseases
CiteScore
2.00
自引率
0.00%
发文量
48
期刊介绍: The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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