脱细胞羊膜及华氏胶对实验性皮肤创面愈合的影响。

IF 4 Q2 MEDICINE, RESEARCH & EXPERIMENTAL
Aline L Takejima, Rossana B Simeoni, Milka L Takejima, Angela G Lemke, Seigo Nagashima, Anna C F Silva, Julio C Francisco, Ricardo A Pinho, Lúcia de Noronha, Luiz C Guarita-Souza
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引用次数: 0

摘要

一些研究聚焦于广泛皮肤病变的新治疗策略,旨在提高愈合质量和缩短治疗时间。在这种情况下,使用羊膜(AM)和沃顿果冻(WJ)成为有希望的替代品。取21只Wistar大鼠背部全层皮肤创面,随机分为3组:对照组(C)、AM -覆盖AM组和WJ -覆盖WJ组。每周测量伤口收缩率。第28天进行基质金属蛋白酶-9 (MMP-9)、转化生长因子-β (TGF-β)和α-平滑肌肌动蛋白(α-SMA)的组织化学和免疫组织化学分析。第7天,AM组创面收缩率最高(38.8%),WJ组次之(27.4%),对照组次之(26.5%),差异有统计学意义。在前14天,AM组保持最高的收缩率,其次是对照组和WJ组。但到了第21天,对照组创面收缩率从WJ到AM依次升高(85.6%、87.0%、91.1%),差异均有统计学意义。I型胶原蛋白在所有组中均占主导地位,各组间差异无统计学意义。TGF-β表达量在WJ、AM组显著高于对照组(19.75%、26.00%、36.56%),差异均有统计学意义。MMP-9和α-SMA的表达在对照组、WJ组和AM组均有所下降,但差异不显著。AM和WJ都增强了早期伤口收缩,并可能通过减弱纤维化信号来支持皮肤修复。这些发现突出了AM和WJ作为生物材料在上皮屏障处促进组织再生的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effects of decellularized amniotic membrane and Wharton's jelly on the healing of experimental skin wounds in rats.

Several studies have focused on novel therapeutic strategies for extensive skin lesions aiming to improve healing quality and reduce treatment duration. In this context, the use of amniotic membrane (AM) and Wharton's jelly (WJ) emerges as promising alternatives. Full-thickness dorsal skin wounds were created in 21 Wistar rats, randomly divided into three groups: control (C), AM - covered by AM and WJ - covered by WJ. Wound contraction rate was measured weekly. On day 28, histochemical (Picrosirius red) and immunohistochemical analyses matrix metalloproteinase-9 (MMP-9), transforming growth factor beta (TGF-β), and alpha-smooth muscle actin (α-SMA) were performed. On day seven, wound contraction rate was higher in the AM group (38.8%), followed by the WJ (27.4%) and control (26.5%) with statistically significance. During the first 14 days, the AM group maintained the highest contraction rate, followed by the control and WJ groups. However, by day 21, wound contraction rates increased in the order of WJ to AM to control groups (85.6%, 87.0%, and 91.1%) with statistically significance. Type I collagen predominated across all groups, without statistically significant differences among them. TGF-β expression significantly increased from WJ to AM to control groups (19.75%, 26.00%, and 36.56%) with statistically significance. MMP-9 and α-SMA expressions decreased from control to WJ to AM groups, but no significant differences were observed. Both AM and WJ enhanced early wound contraction and may support skin repair by attenuating fibrotic signaling. These findings highlight the potential of AM and WJ as biomaterials for promoting tissue regeneration at epithelial barriers.

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来源期刊
Tissue Barriers
Tissue Barriers MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
6.60
自引率
6.50%
发文量
25
期刊介绍: Tissue Barriers is the first international interdisciplinary journal that focuses on the architecture, biological roles and regulation of tissue barriers and intercellular junctions. We publish high quality peer-reviewed articles that cover a wide range of topics including structure and functions of the diverse and complex tissue barriers that occur across tissue and cell types, including the molecular composition and dynamics of polarized cell junctions and cell-cell interactions during normal homeostasis, injury and disease state. Tissue barrier formation in regenerative medicine and restoration of tissue and organ function is also of interest. Tissue Barriers publishes several categories of articles including: Original Research Papers, Short Communications, Technical Papers, Reviews, Perspectives and Commentaries, Hypothesis and Meeting Reports. Reviews and Perspectives/Commentaries will typically be invited. We also anticipate to publish special issues that are devoted to rapidly developing or controversial areas of research. Suggestions for topics are welcome. Tissue Barriers objectives: Promote interdisciplinary awareness and collaboration between researchers working with epithelial, epidermal and endothelial barriers and to build a broad and cohesive worldwide community of scientists interesting in this exciting field. Comprehend the enormous complexity of tissue barriers and map cross-talks and interactions between their different cellular and non-cellular components. Highlight the roles of tissue barrier dysfunctions in human diseases. Promote understanding and strategies for restoration of tissue barrier formation and function in regenerative medicine. Accelerate a search for pharmacological enhancers of tissue barriers as potential therapeutic agents. Understand and optimize drug delivery across epithelial and endothelial barriers.
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