Hong-Ming Fan, Le-Min Hong, Chun-Qun Huang, Jin-Feng Lu, Hong-Hui Xu, Jie Chen, Hong-Ming Huang, Xin-Feng Wang, Dan Guo
{"title":"TBL1XR1突变对弥漫性大b细胞淋巴瘤细胞生物学特性的影响","authors":"Hong-Ming Fan, Le-Min Hong, Chun-Qun Huang, Jin-Feng Lu, Hong-Hui Xu, Jie Chen, Hong-Ming Huang, Xin-Feng Wang, Dan Guo","doi":"10.19746/j.cnki.issn.1009-2137.2025.02.016","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effect of <i>TBL1XR1</i> mutation on cell biological characteristics of diffuse large B-cell lymphoma (DLBCL).</p><p><strong>Methods: </strong>The <i>TBL1XR1</i> overexpression vector was constructed and DNA sequencing was performed to determine the mutation status. The effect of <i>TBL1XR1</i> mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay; CCK-8 assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell proliferation; Transwell assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell migration and invasion; Western blot was used to detect the effect of <i>TBL1XR1</i> mutation on the expression level of epithelial-mesenchymal transition (EMT) related proteins.</p><p><strong>Results: </strong>The <i>TBL1XR1</i> overexpression plasmid was successfully constructed. The <i>in vitro</i> experimental results showed that <i>TBL1XR1</i> mutation had no significant effect on apoptosis of DLBCL cells. Compared with the control group, <i>TBL1XR1</i> mutation enhanced cell proliferation, migration and invasion of DLBCL cells. <i>TBL1XR1</i> gene mutation significantly increased the expression of N-cadherin protein, while the expression of E-cadherin protein decreased.</p><p><strong>Conclusion: </strong><i>TBL1XR1</i> mutation plays a role in promoting tumor cell proliferation, migration and invasion in DLBCL. <i>TBL1XR1</i> could be considered as a potential target for DLBCL therapy in future research.</p>","PeriodicalId":35777,"journal":{"name":"中国实验血液学杂志","volume":"33 2","pages":"423-430"},"PeriodicalIF":0.0000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Effect of <i>TBL1XR1</i> Mutation on Cell Biological Characteristics of Diffuse Large B-Cell Lymphoma].\",\"authors\":\"Hong-Ming Fan, Le-Min Hong, Chun-Qun Huang, Jin-Feng Lu, Hong-Hui Xu, Jie Chen, Hong-Ming Huang, Xin-Feng Wang, Dan Guo\",\"doi\":\"10.19746/j.cnki.issn.1009-2137.2025.02.016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the effect of <i>TBL1XR1</i> mutation on cell biological characteristics of diffuse large B-cell lymphoma (DLBCL).</p><p><strong>Methods: </strong>The <i>TBL1XR1</i> overexpression vector was constructed and DNA sequencing was performed to determine the mutation status. The effect of <i>TBL1XR1</i> mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay; CCK-8 assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell proliferation; Transwell assay was used to detect the effect of <i>TBL1XR1</i> mutation on cell migration and invasion; Western blot was used to detect the effect of <i>TBL1XR1</i> mutation on the expression level of epithelial-mesenchymal transition (EMT) related proteins.</p><p><strong>Results: </strong>The <i>TBL1XR1</i> overexpression plasmid was successfully constructed. The <i>in vitro</i> experimental results showed that <i>TBL1XR1</i> mutation had no significant effect on apoptosis of DLBCL cells. Compared with the control group, <i>TBL1XR1</i> mutation enhanced cell proliferation, migration and invasion of DLBCL cells. <i>TBL1XR1</i> gene mutation significantly increased the expression of N-cadherin protein, while the expression of E-cadherin protein decreased.</p><p><strong>Conclusion: </strong><i>TBL1XR1</i> mutation plays a role in promoting tumor cell proliferation, migration and invasion in DLBCL. <i>TBL1XR1</i> could be considered as a potential target for DLBCL therapy in future research.</p>\",\"PeriodicalId\":35777,\"journal\":{\"name\":\"中国实验血液学杂志\",\"volume\":\"33 2\",\"pages\":\"423-430\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国实验血液学杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.016\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国实验血液学杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.19746/j.cnki.issn.1009-2137.2025.02.016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
[Effect of TBL1XR1 Mutation on Cell Biological Characteristics of Diffuse Large B-Cell Lymphoma].
Objective: To investigate the effect of TBL1XR1 mutation on cell biological characteristics of diffuse large B-cell lymphoma (DLBCL).
Methods: The TBL1XR1 overexpression vector was constructed and DNA sequencing was performed to determine the mutation status. The effect of TBL1XR1 mutation on apoptosis of DLBCL cell line was detected by flow cytometry and TUNEL fluorescence assay; CCK-8 assay was used to detect the effect of TBL1XR1 mutation on cell proliferation; Transwell assay was used to detect the effect of TBL1XR1 mutation on cell migration and invasion; Western blot was used to detect the effect of TBL1XR1 mutation on the expression level of epithelial-mesenchymal transition (EMT) related proteins.
Results: The TBL1XR1 overexpression plasmid was successfully constructed. The in vitro experimental results showed that TBL1XR1 mutation had no significant effect on apoptosis of DLBCL cells. Compared with the control group, TBL1XR1 mutation enhanced cell proliferation, migration and invasion of DLBCL cells. TBL1XR1 gene mutation significantly increased the expression of N-cadherin protein, while the expression of E-cadherin protein decreased.
Conclusion: TBL1XR1 mutation plays a role in promoting tumor cell proliferation, migration and invasion in DLBCL. TBL1XR1 could be considered as a potential target for DLBCL therapy in future research.
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