利拉鲁肽对胰腺β细胞胰岛素分泌和β细胞存活相关GPCR基因表达的影响。

IF 1.2 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Turkish Journal of Medical Sciences Pub Date : 2025-03-20 eCollection Date: 2025-01-01 DOI:10.55730/1300-0144.5997
Melikenur Türkol, Türker Bilgen
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引用次数: 0

摘要

背景/目的:利拉鲁肽是一种长效胰高血糖素样肽类药物,具有抗高血糖和抗肥胖作用。G蛋白偶联受体(gpcr)是众所周知的药物靶标分子,其介导与疾病相关的关键信号通路。研究证实了许多gpcr基因在调节胰腺β细胞功能和胰岛素分泌中的作用。利拉鲁肽依赖性GPCR基因的表达变化可能让我们确定新的药物靶点。材料和方法:因此,我们在本研究中研究了GPCR家族成员GPR75、GPR56、GLP1R、M3R和CB1R基因在利拉鲁肽处理后在nit1小鼠胰腺β细胞中的表达变化。采用qPCR和ddCT方法,并采用对照基因和未处理对照组,检测这些GPCR基因mRNA水平的变化。结果:我们发现10nM利拉鲁肽在60 min时GPR75、GPR56、M3R和CB1R基因mRNA水平有统计学意义的升高,而所有研究的基因都没有时间和剂量依赖性的变化。我们检测到GLP1R基因表达在不同时间点和剂量的利拉鲁肽中都是稳定的,除了1000nM利拉鲁肽处理的GLP1R基因表达比10nM和100nM浓度的GLP1R基因表达有统计学意义上的降低。结论:体外利拉鲁肽处理胰岛β细胞可增加与胰岛素分泌和β细胞存活相关的GPR75、GPR56、M3R和CB1R基因的表达。利拉鲁肽可能通过GLP1R或其他尚未描述的细胞途径发挥这种作用。利拉鲁肽与GPR75、GPR56、M3R和CB1R特异性配体联合使用可能在胰岛素分泌和β细胞存活方面提供更好的应答,使其成为抗糖尿病和抗肥胖治疗的良好靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effects of liraglutide on expressions of insulin secretion and beta cell survive associated GPCR genes in pancreatic beta cells.

Background/aim: Liraglutide as a long-acting glucagon-like peptide drug has anti-hyperglycemic and antiobesity effects. G protein-coupled receptors (GPCRs) are well-known drug target molecules that conduct critical signaling pathways related with diseases. Research has confirmed the role of many GPCRs genes in the regulation of pancreatic beta cell functions and insulin secretion. Liraglutide dependent expressional changes in GPCR genes may let us determine new drug targets.

Materials and methods: Therefore, we investigated the changes in expression of GPR75, GPR56, GLP1R, M3R, and CB1R genes, which are the GPCR family members, in response to liraglutide treatment in the NIT-1 mouse pancreatic beta cells in this study. Changes at the mRNA levels of these GPCR genes were determined by a qPCR and the ddCT method, and using a control gene and untreated control groups.

Results: We found statistically significant increases at the mRNA levels of GPR75, GPR56, M3R, and CB1R genes with 10nM of liraglutide at min 60, while there was no time and dose-dependent change in all of the genes investigated. We detected that the GLP1R gene expressions were stable amongst different time points and doses of liraglutide, except for a statistically significant decrease in the GLP1R gene expression in response to 1000nM of liraglutide treatment compared to 10nM and 100nM concentrations.

Conclusion: Our results indicate that in vitro liraglutide administration in pancreatic beta cells appears to increase the expressions of GPR75, GPR56, M3R and CB1R genes which have already been related to insulin secretion and beta cell survive. Liraglutide may exert this effect through the GLP1R or other cellular pathways undescribed yet. Combined usage of liraglutide and the specific ligands of GPR75, GPR56, M3R, and CB1R may provide a better response in terms of insulin secretion and beta cell survival, making them good targets for antidiabetic and antiobesity therapy.

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来源期刊
Turkish Journal of Medical Sciences
Turkish Journal of Medical Sciences 医学-医学:内科
CiteScore
4.60
自引率
4.30%
发文量
143
审稿时长
3-8 weeks
期刊介绍: Turkish Journal of Medical sciences is a peer-reviewed comprehensive resource that provides critical up-to-date information on the broad spectrum of general medical sciences. The Journal intended to publish original medical scientific papers regarding the priority based on the prominence, significance, and timeliness of the findings. However since the audience of the Journal is not limited to any subspeciality in a wide variety of medical disciplines, the papers focusing on the technical  details of a given medical  subspeciality may not be evaluated for publication.
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