{"title":"大鼠泪腺肥大细胞的组织化学和免疫组织化学鉴定。","authors":"Ozkan Yavas","doi":"10.1111/vop.70005","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study aims to investigate the presence, microanatomical localization, and immunohistochemical subtypes of mast cells in the lacrimal glands (LGs) of rats.</p><p><strong>Animals: </strong>A total of ten female Sprague-Dawley rats' LGs were evaluated in this study.</p><p><strong>Procedure(s): </strong>The experimental animals were euthanized. LG samples were collected and fixed in 10% neutral formaldehyde for 48 h. Sections were taken from the paraffin-embedded samples and stained for microscopic examination. The histochemical properties of lacrimal gland epithelium and the composition of tears were examined by Periodic acid-Schiff and Alcian blue staining and evaluated microscopically. Toluidine blue staining and tryptase and chymase immunohistochemically demonstrated localization and phenotypes of mast cells.</p><p><strong>Results: </strong>The existence and varying numbers of mast cells were successfully demonstrated in all three LGs by Toluidine blue staining, and the highest number of mast cells was observed in the extraorbital lacrimal gland, while their numbers were almost the same in the intraorbital lacrimal gland and Harderian gland. Immunohistochemically, chymase-positive mast cells were more numerous than tryptase-positive cells.</p><p><strong>Conclusions: </strong>This study is the first documentation of the presence of mast cells with different phenotypes in rat LGs.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Histochemical and Immunohistochemical Identification of Mast Cells in the Rat Lacrimal Glands.\",\"authors\":\"Ozkan Yavas\",\"doi\":\"10.1111/vop.70005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study aims to investigate the presence, microanatomical localization, and immunohistochemical subtypes of mast cells in the lacrimal glands (LGs) of rats.</p><p><strong>Animals: </strong>A total of ten female Sprague-Dawley rats' LGs were evaluated in this study.</p><p><strong>Procedure(s): </strong>The experimental animals were euthanized. LG samples were collected and fixed in 10% neutral formaldehyde for 48 h. Sections were taken from the paraffin-embedded samples and stained for microscopic examination. The histochemical properties of lacrimal gland epithelium and the composition of tears were examined by Periodic acid-Schiff and Alcian blue staining and evaluated microscopically. Toluidine blue staining and tryptase and chymase immunohistochemically demonstrated localization and phenotypes of mast cells.</p><p><strong>Results: </strong>The existence and varying numbers of mast cells were successfully demonstrated in all three LGs by Toluidine blue staining, and the highest number of mast cells was observed in the extraorbital lacrimal gland, while their numbers were almost the same in the intraorbital lacrimal gland and Harderian gland. Immunohistochemically, chymase-positive mast cells were more numerous than tryptase-positive cells.</p><p><strong>Conclusions: </strong>This study is the first documentation of the presence of mast cells with different phenotypes in rat LGs.</p>\",\"PeriodicalId\":23836,\"journal\":{\"name\":\"Veterinary ophthalmology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2025-04-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinary ophthalmology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1111/vop.70005\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary ophthalmology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/vop.70005","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Histochemical and Immunohistochemical Identification of Mast Cells in the Rat Lacrimal Glands.
Objective: This study aims to investigate the presence, microanatomical localization, and immunohistochemical subtypes of mast cells in the lacrimal glands (LGs) of rats.
Animals: A total of ten female Sprague-Dawley rats' LGs were evaluated in this study.
Procedure(s): The experimental animals were euthanized. LG samples were collected and fixed in 10% neutral formaldehyde for 48 h. Sections were taken from the paraffin-embedded samples and stained for microscopic examination. The histochemical properties of lacrimal gland epithelium and the composition of tears were examined by Periodic acid-Schiff and Alcian blue staining and evaluated microscopically. Toluidine blue staining and tryptase and chymase immunohistochemically demonstrated localization and phenotypes of mast cells.
Results: The existence and varying numbers of mast cells were successfully demonstrated in all three LGs by Toluidine blue staining, and the highest number of mast cells was observed in the extraorbital lacrimal gland, while their numbers were almost the same in the intraorbital lacrimal gland and Harderian gland. Immunohistochemically, chymase-positive mast cells were more numerous than tryptase-positive cells.
Conclusions: This study is the first documentation of the presence of mast cells with different phenotypes in rat LGs.
期刊介绍：
Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of:
Clinical and investigational veterinary and comparative ophthalmology;
Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species;
Experimental models of both animal and human ocular disease in veterinary species;
Anatomic studies of the animal eye;
Physiological studies of the animal eye;
Pharmacological studies of the animal eye.
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