刺花椒和辣木叶片对乳腺组织Her2、Cox-2和Caspase 3表达的影响

Q3 Agricultural and Biological Sciences

Pakistan Journal of Biological Sciences Pub Date : 2025-03-01 DOI:10.3923/pjbs.2025.219.228

Rostime Hermayerni Simanullang, Masta Melati Hutahaean, Jekson Martiar Siahaan, Calen, Kasrawati, Ganda Sigalingging, Hadiyanto Lim, Putri Cahaya Situmorang

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Materials and Methods: This study used 36 rats with 6 groups, namely C0: Normal mice, C: Only DMBA injection, C+: DMBA-injected rats+Doxorubicin, P1: DMBA-injected rats+Z. acanthopodium fruit, P2: DMBA-injected rats+100 mg/kg b.wt., of Moringa oleifera leaves and DMBA-injected rats+50 mg/kg b.wt., of Moringa oleifera leaves and 50 mg/kg b.wt., of Z. acanthopodium fruit orally for 30 days then dissected and breast tissue was taken for immunohistochemistry procedures. The non-parametric data were analyzed using the Kruskal-Wallis and Mann-Whitney tests with a significance level of p<0.05. Results: The DMBA injection into the mammary tissue increases Her2 expression, infiltrates the surrounding tissue, including the stroma and forms a solid tumor mass with ambiguous boundaries. Zanthoxylum acanthopodium and Moringa oleifera alone reduced damaged tissue, however, their combined action boosted breast tissue Her2 expression but did not lower Cox-2 or Caspase 3 scores. 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引用次数: 0

摘要

背景和目标：<；/b>；乳房组织可能由于感染、疾病、炎症或暴露于有害化合物等因素而发生结构改变，7,12-二甲基苯[a]蒽（DMBA）是癌症研究中常用的致癌物。& lt; i>花椒属植物acanthopodiumDC ；辣木<；/i>；叶子具有抗炎和抗癌作用。本研究旨在检测给药后Her2、Cox-2和Caspase 3作为乳腺癌标志物的表达情况。DC ；辣木<；/i>；叶子。材料和方法：<；/b>；本研究选用36只大鼠，分为6组，即C0：正常小鼠，C；：仅注射DMBA， C+；：注射DMBA大鼠+阿霉素，P1：注射DMBA大鼠+<；i>；Z。acanthopodium水果，P2: dba注射大鼠+100 mg/kg b.wt。辣木<；/i>；叶片和dba注射大鼠+50 mg/kg b.wt。辣木<；/i>；叶片和50毫克/公斤重量。；；；；；acanthopodium水果口服30天，然后解剖并取乳腺组织进行免疫组织化学处理。非参数数据采用Kruskal-Wallis检验和Mann-Whitney检验进行分析，显著性水平为p

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Histological Changes in Breast Tissue via the Expression of Her2, Cox-2 and Caspase 3 after the Administration of Zanthoxylum acanthopodium DC and Moringa oleifera Leaves.

Background and Objective: Breast tissue may experience structural alterations due to variables including infection, illness, inflammation, or exposure to deleterious compounds, with 7,12-Dimethylbenz[a]anthracene (DMBA) being a prevalent carcinogen utilized in cancer research. Zanthoxylum acanthopodium DC and Moringa oleifera leaves possess anti-inflammatory and anticancer effects. This study aimed to determine the expression of Her2, Cox-2 and Caspase 3 as markers of breast carcinoma after administration of Zanthoxylum acanthopodium DC and Moringa oleifera leaves. Materials and Methods: This study used 36 rats with 6 groups, namely C0: Normal mice, C: Only DMBA injection, C+: DMBA-injected rats+Doxorubicin, P1: DMBA-injected rats+Z. acanthopodium fruit, P2: DMBA-injected rats+100 mg/kg b.wt., of Moringa oleifera leaves and DMBA-injected rats+50 mg/kg b.wt., of Moringa oleifera leaves and 50 mg/kg b.wt., of Z. acanthopodium fruit orally for 30 days then dissected and breast tissue was taken for immunohistochemistry procedures. The non-parametric data were analyzed using the Kruskal-Wallis and Mann-Whitney tests with a significance level of p<0.05. Results: The DMBA injection into the mammary tissue increases Her2 expression, infiltrates the surrounding tissue, including the stroma and forms a solid tumor mass with ambiguous boundaries. Zanthoxylum acanthopodium and Moringa oleifera alone reduced damaged tissue, however, their combined action boosted breast tissue Her2 expression but did not lower Cox-2 or Caspase 3 scores. The expression of Cox-2 in group C differed significantly from P1 (p<0.05, p = 0.001). Conclusion: Zanthoxylum acanthopodium and Moringa oleifera enhanced Caspase 3, which promotes apoptosis in breast cancer cells. Nevertheless, each plant individually diminished Her2 and Cox-2 expression more effectively than the combination.

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来源期刊

Pakistan Journal of Biological Sciences Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

1.90

自引率

0.00%

发文量

102

期刊介绍： Pakistan Journal of Biological Sciences (PJBS) is an international, peer-reviewed and well indexed scientific journal seeks to promote and disseminate the knowledge of biological sciences by publishing outstanding research in the field. Scope of the journal includes: Cell biology, developmental biology, structural biology, microbiology, entomology, toxicology, molecular biology & genetics, biochemistry, biotechnology, biodiversity, ecology, marine biology, plant biology and bioinformatics.