副假丝酵母生物膜对杀菌剂的细胞和物质特异性反应。

IF 3.9 3区医学 Q1 INFECTIOUS DISEASES

Journal of Hospital Infection Pub Date : 2025-05-06 DOI:10.1016/j.jhin.2025.04.023

J.K.S. Dourado , C.M. Álvarez-Ormeño , G. Benard , R.H. Pires

{"title":"副假丝酵母生物膜对杀菌剂的细胞和物质特异性反应。","authors":"J.K.S. Dourado , C.M. Álvarez-Ormeño , G. Benard , R.H. Pires","doi":"10.1016/j.jhin.2025.04.023","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div><em>Candida parapsilosis</em> biofilms pose significant challenges in clinical settings due to their resilience to biocides and the presence of persister cells. This study evaluated the efficacy of amphotericin B (AmB), chlorhexidine (CLX), and ortho-phthalaldehyde (OPA) against biofilms formed by fluconazole-resistant <em>C. parapsilosis</em> isolates and examined the role of persister cells and catheter materials in biofilm resilience.</div></div><div><h3>Methods</h3><div>Clinical isolates of <em>C. parapsilosis</em> from hospital surfaces were exposed to CLX (0.5%) and OPA (0.55%). Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Biofilm susceptibility was assessed using the crystal violet assay and total plate counts. Scanning electron microscopy (SEM) analysed biofilm structural changes on different catheter materials.</div></div><div><h3>Findings</h3><div>AmB demonstrated high efficacy with low MIC and MFC values. CLX showed moderate efficacy, with higher MICs and MFCs in clinical isolates compared with the standard strain. OPA exhibited the lowest efficacy, with higher MIC, MFC, and SMIC<sub>80</sub> values. Persister cells were confirmed in isolate CBL 1031, while isolates 17D, 26E, and ATCC 90019 displayed significant SMIC80 reductions, indicating high susceptibility. PTFE materials showed significant biofilm reductions post-biocide exposure, while HDPE displayed the greatest biofilm resilience with higher residual cell counts. SEM revealed material-specific biofilm disruption, with greater structural damage on HDPE and silicone-coated latex (SCL) compared with PTFE.</div></div><div><h3>Conclusions</h3><div>These findings underscore the importance of optimizing cleaning protocols, selecting appropriate medical materials, and addressing persister cells to mitigate biofilm-related infections in healthcare settings.</div></div>","PeriodicalId":54806,"journal":{"name":"Journal of Hospital Infection","volume":"162 ","pages":"Pages 84-94"},"PeriodicalIF":3.9000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cellular and material-specific responses of Candida parapsilosis biofilms to biocides\",\"authors\":\"J.K.S. Dourado , C.M. Álvarez-Ormeño , G. Benard , R.H. Pires\",\"doi\":\"10.1016/j.jhin.2025.04.023\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div><em>Candida parapsilosis</em> biofilms pose significant challenges in clinical settings due to their resilience to biocides and the presence of persister cells. This study evaluated the efficacy of amphotericin B (AmB), chlorhexidine (CLX), and ortho-phthalaldehyde (OPA) against biofilms formed by fluconazole-resistant <em>C. parapsilosis</em> isolates and examined the role of persister cells and catheter materials in biofilm resilience.</div></div><div><h3>Methods</h3><div>Clinical isolates of <em>C. parapsilosis</em> from hospital surfaces were exposed to CLX (0.5%) and OPA (0.55%). Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Biofilm susceptibility was assessed using the crystal violet assay and total plate counts. Scanning electron microscopy (SEM) analysed biofilm structural changes on different catheter materials.</div></div><div><h3>Findings</h3><div>AmB demonstrated high efficacy with low MIC and MFC values. CLX showed moderate efficacy, with higher MICs and MFCs in clinical isolates compared with the standard strain. OPA exhibited the lowest efficacy, with higher MIC, MFC, and SMIC<sub>80</sub> values. Persister cells were confirmed in isolate CBL 1031, while isolates 17D, 26E, and ATCC 90019 displayed significant SMIC80 reductions, indicating high susceptibility. PTFE materials showed significant biofilm reductions post-biocide exposure, while HDPE displayed the greatest biofilm resilience with higher residual cell counts. SEM revealed material-specific biofilm disruption, with greater structural damage on HDPE and silicone-coated latex (SCL) compared with PTFE.</div></div><div><h3>Conclusions</h3><div>These findings underscore the importance of optimizing cleaning protocols, selecting appropriate medical materials, and addressing persister cells to mitigate biofilm-related infections in healthcare settings.</div></div>\",\"PeriodicalId\":54806,\"journal\":{\"name\":\"Journal of Hospital Infection\",\"volume\":\"162 \",\"pages\":\"Pages 84-94\"},\"PeriodicalIF\":3.9000,\"publicationDate\":\"2025-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Hospital Infection\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0195670125001252\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Hospital Infection","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0195670125001252","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

摘要

背景：假丝酵母假丝病生物膜由于其对杀菌剂的弹性和持久性细胞的存在，在临床环境中提出了重大挑战。本研究评估了两性霉素B （AmB）、氯己定（CLX）和邻苯二醛（OPA）对氟康唑耐药C. parapsilosis分离株形成的生物膜的作用，并研究了持久性细胞和导管材料在生物膜弹性中的作用。方法：将医院表面分离的假梭菌分别暴露于氯己定（0.5%）和邻苯二醛（0.55%）中。用微量肉汤稀释法测定最低抑菌浓度（MIC）。用结晶紫法和总平板计数评估生物膜敏感性。扫描电镜（SEM）分析了不同导管材料上生物膜结构的变化。结果：AmB具有较低MIC和MFC值的高疗效。CLX表现出中等疗效，与标准菌株相比，临床分离株的mic和mfc更高。OPA的疗效最低，MIC、MFC和SMIC80值较高。分离物CBL 1031中证实了持久性细胞，而分离物17D、26E和ATCC 90019显示SMIC80显著减少，表明高敏感性。PTFE材料在杀菌剂暴露后表现出明显的生物膜减少，而HDPE材料表现出最大的生物膜恢复能力，残留细胞计数较高。扫描电镜显示材料特异性生物膜破坏，与PTFE相比，HDPE和硅涂层乳胶（SCL）的结构损伤更大。结论：这些发现强调了优化清洁方案、选择合适的医疗材料和处理持久性细胞以减轻医疗机构中生物膜相关感染的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Cellular and material-specific responses of Candida parapsilosis biofilms to biocides

Background

Candida parapsilosis biofilms pose significant challenges in clinical settings due to their resilience to biocides and the presence of persister cells. This study evaluated the efficacy of amphotericin B (AmB), chlorhexidine (CLX), and ortho-phthalaldehyde (OPA) against biofilms formed by fluconazole-resistant C. parapsilosis isolates and examined the role of persister cells and catheter materials in biofilm resilience.

Methods

Clinical isolates of C. parapsilosis from hospital surfaces were exposed to CLX (0.5%) and OPA (0.55%). Minimum inhibitory concentrations (MICs) were determined by broth microdilution. Biofilm susceptibility was assessed using the crystal violet assay and total plate counts. Scanning electron microscopy (SEM) analysed biofilm structural changes on different catheter materials.

Findings

AmB demonstrated high efficacy with low MIC and MFC values. CLX showed moderate efficacy, with higher MICs and MFCs in clinical isolates compared with the standard strain. OPA exhibited the lowest efficacy, with higher MIC, MFC, and SMIC₈₀ values. Persister cells were confirmed in isolate CBL 1031, while isolates 17D, 26E, and ATCC 90019 displayed significant SMIC80 reductions, indicating high susceptibility. PTFE materials showed significant biofilm reductions post-biocide exposure, while HDPE displayed the greatest biofilm resilience with higher residual cell counts. SEM revealed material-specific biofilm disruption, with greater structural damage on HDPE and silicone-coated latex (SCL) compared with PTFE.

Conclusions

These findings underscore the importance of optimizing cleaning protocols, selecting appropriate medical materials, and addressing persister cells to mitigate biofilm-related infections in healthcare settings.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Hospital Infection 医学-传染病学

CiteScore

12.70

自引率

5.80%

发文量

271

审稿时长

19 days

期刊介绍： The Journal of Hospital Infection is the editorially independent scientific publication of the Healthcare Infection Society. The aim of the Journal is to publish high quality research and information relating to infection prevention and control that is relevant to an international audience. The Journal welcomes submissions that relate to all aspects of infection prevention and control in healthcare settings. This includes submissions that: provide new insight into the epidemiology, surveillance, or prevention and control of healthcare-associated infections and antimicrobial resistance in healthcare settings; provide new insight into cleaning, disinfection and decontamination; provide new insight into the design of healthcare premises; describe novel aspects of outbreaks of infection; throw light on techniques for effective antimicrobial stewardship; describe novel techniques (laboratory-based or point of care) for the detection of infection or antimicrobial resistance in the healthcare setting, particularly if these can be used to facilitate infection prevention and control; improve understanding of the motivations of safe healthcare behaviour, or describe techniques for achieving behavioural and cultural change; improve understanding of the use of IT systems in infection surveillance and prevention and control.