Safety and hemostatic effect of Achillea millefolium L. in localized bleeding.

Background and aim: The objectives of the study were to present the hemostatic effect of hydroalcoholic extract of Achillea millefolium L. in localized bleeding and to evaluate the safety of this plant after topical usage in rat's liver.

Materials and methods: The aerial parts of this plant were macerated for 2 days using methanol. After anesthesia and laparotomy of 12 female Wistar rats (120-220 g), the liver was exposed and two incisions were performed for bleeding. One was packed by sponge with A. millefolium and another without A. millefolium as a control group. Animals were divided into two groups that A. millefolium (150 mg/kg) was used in the first incision for one group and in the second incision for another. Liver biopsy was taken after 4, 6, and 8 weeks.

Results: We observed that usage of A. millefolium for liver incisions, either in the first incision or in the second incision; bleeding time decreases significantly (36.1% and 31.9%, respectively). Histopathological evaluations revealed no signs of toxic and hepatic damage for periods 4, 6, and 8 weeks in the female rats.

Conclusion: This study confirmed the hemostatic effect of hydroalcoholic extract of A. millefolium in localized bleeding and also the safety of this plant for topical usage.