叶绿素代谢基因在马铃薯块茎光诱导变绿中的相对重要性。

IF 2.1 4区 生物学 Q2 PLANT SCIENCES
Photosynthetica Pub Date : 2025-02-13 eCollection Date: 2025-01-01 DOI:10.32615/ps.2025.003
S Tanios, T Thangavel, A Eyles, R S Tegg, C R Wilson
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引用次数: 0

摘要

马铃薯块茎的变绿是由于光照下叶绿素的积累而发生的,然而,关于块茎叶绿素代谢的基本信息缺乏。我们测量了不同光照(0、48、96和168 h)对不同绿色化倾向马铃薯品种叶绿素浓度和叶绿素代谢途径酶基因表达的影响。绿化与参与叶绿素生物合成的基因,特别是谷氨酰trna还原酶1、镁螯合酶亚基H和镁原卟啉IX单甲基酯环化酶的上调有关,与叶绿素循环和降解相关的基因,包括叶绿素内酯加氧酶和叶绿素内酯加氧酶的下调有关。我们的研究结果表明,对块茎变绿倾向的相对抗性可能是由于叶绿素生物合成基因的上调较弱,叶绿素降解基因的下调较弱。这些生物合成和降解基因与绿化敏感性的关联可能为未来培育更多的绿色抗性品种提供可能的育种靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Relative importance of chlorophyll metabolic genes for light-induced greening of potato tubers.

Potato tuber greening occurs due to the chlorophyll accumulation upon exposure to light, however, fundamental information on tuber chlorophyll metabolism is lacking. We measured the effect of varying light exposure (0, 48, 96, and 168 h) on chlorophyll concentration and gene expression of enzymes in the chlorophyll metabolic pathway in the potato varieties that differ in greening propensity. Greening was associated with the upregulation of genes involved in chlorophyll biosynthesis, particularly glutamyl-tRNA reductase 1, magnesium-chelatase subunit H, and magnesium-protoporphyrin IX monomethyl ester cyclase, and downregulation of genes involved in chlorophyll cycling and degradation, including chlorophyllide a oxygenase, and pheophorbide a oxygenase. Our findings suggest that relative resistance to tuber greening propensity may be due to a weaker upregulation of chlorophyll biosynthesis genes and weaker downregulation of chlorophyll degradation genes that occurs in susceptible varieties. The association of these biosynthesis and degradation genes with greening susceptibility may provide possible breeding targets for the future development of more greening-resistant varieties.

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来源期刊
Photosynthetica
Photosynthetica 生物-植物科学
CiteScore
5.60
自引率
7.40%
发文量
55
审稿时长
3.8 months
期刊介绍: Photosynthetica publishes original scientific papers and brief communications, reviews on specialized topics, book reviews and announcements and reports covering wide range of photosynthesis research or research including photosynthetic parameters of both experimental and theoretical nature and dealing with physiology, biophysics, biochemistry, molecular biology on one side and leaf optics, stress physiology and ecology of photosynthesis on the other side. The language of journal is English (British or American). Papers should not be published or under consideration for publication elsewhere.
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