利用全基因组序列分析和分子网络技术探索内生真菌青霉BLH34的生物合成潜力。

IF 1.9 3区化学 Q3 CHEMISTRY, APPLIED

Natural Product Research Pub Date : 2025-05-10 DOI:10.1080/14786419.2025.2500738

Xuejun Wang, Jun Chen, Han Lin, Changli Min, Li Zhang, Zhimou Gao

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引用次数: 0

摘要

本文对从黄芩中分离得到的内生真菌青霉BLH34进行了深入的基因组学和代谢组学研究。杂交测序（Illumina-Nanopore）产生了高质量的27.9 Mb基因组（GC 48.6%），编码9798个蛋白，功能注释将5350个基因与NCBI非冗余数据库连接，3404个与KEGG通路连接。AntiSMASH分析发现了35个生物合成基因簇（BGCs），其中23个与已知途径缺乏同源性，这表明BLH34有可能发现新的代谢物。分子网络（GNPS）和LC-MS/MS鉴定了19种特殊代谢物，包括抗菌聚酮。生物测定显示对金黄色葡萄球菌（36毫米）、枯草芽孢杆菌（28毫米）和大肠杆菌（24毫米）有有效的抑制作用，强调了其药物相关性。

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Exploring biosynthetic potential of the endophytic Penicillium turbatum BLH34 using whole-genome sequence analysis and molecular networking.

An in-depth genomic and metabolomic investigation was conducted on the endophytic fungus Penicillium turbatum BLH34, isolated from Macleaya cordata. Hybrid sequencing (Illumina-Nanopore) generated a high-quality 27.9 Mb genome (GC 48.6%) encoding 9798 proteins, with functional annotation linking 5350 genes to the NCBI non-redundant database and 3404 to KEGG pathways. AntiSMASH analysis uncovered 35 biosynthetic gene clusters (BGCs), 23 of which lacked homology to known pathways, highlighting BLH34's potential for novel metabolite discovery. Molecular networking (GNPS) and LC-MS/MS identified 19 specialised metabolites, including antimicrobial polyketides. Bioassays demonstrated potent inhibition against Staphylococcus aureus (36 mm), Bacillus subtilis (28 mm) and Escherichia coli (24 mm), underscoring its pharmaceutical relevance.

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来源期刊

Natural Product Research 化学-医药化学

CiteScore

5.10

自引率

9.10%

发文量

605

审稿时长

2.1 months

期刊介绍： The aim of Natural Product Research is to publish important contributions in the field of natural product chemistry. The journal covers all aspects of research in the chemistry and biochemistry of naturally occurring compounds. The communications include coverage of work on natural substances of land and sea and of plants, microbes and animals. Discussions of structure elucidation, synthesis and experimental biosynthesis of natural products as well as developments of methods in these areas are welcomed in the journal. Finally, research papers in fields on the chemistry-biology boundary, eg. fermentation chemistry, plant tissue culture investigations etc., are accepted into the journal. Natural Product Research issues will be subtitled either ""Part A - Synthesis and Structure"" or ""Part B - Bioactive Natural Products"". for details on this , see the forthcoming articles section. All manuscript submissions are subject to initial appraisal by the Editor, and, if found suitable for further consideration, to peer review by independent, anonymous expert referees. All peer review is single blind and submission is online via ScholarOne Manuscripts.