Zhiming Kang, Wenjing Lei, Ji Pu, Lijun Zhao, Guowen Min, Yufeng Liu, Wenjuan Chang, Yuqian Gao, Kui Dong, Bin Sun
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Genome-based phylogeny demonstrated that <i>Roseomonas harenae</i> CPCC 101081<sup>T</sup> and '<i>R. chloroacetimidivorans</i>' BUT-13<sup>T</sup>species are close relatives to strains SXEYE001<sup>T</sup> and SXEYE002. The average nucleotide identity between the strains and species of the genus <i>Roseomonas</i> was <95.0%. The G+C contents of strains SXEYE001<sup>T</sup> and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001<sup>T</sup> and SXEYE002 were indicative of a novel species within the genus <i>Roseomonas</i>, for which the name <i>Roseomonas xinghualingensis</i> sp. nov. is proposed, with SXEYE001<sup>T</sup> (=GDMCC 1.3626<sup>T</sup>=JCM 35902<sup>T</sup>) as the type strain.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 5","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of <i>Roseomonas xinghualingensis</i> sp. nov. isolated from hospital air.\",\"authors\":\"Zhiming Kang, Wenjing Lei, Ji Pu, Lijun Zhao, Guowen Min, Yufeng Liu, Wenjuan Chang, Yuqian Gao, Kui Dong, Bin Sun\",\"doi\":\"10.1099/ijsem.0.006775\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The genus <i>Roseomonas</i> comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of <i>Roseomonas</i> remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001<sup>T</sup> and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to '<i>Roseomonas chloroacetimidivorans</i>' BUT 13<sup>T</sup>. The 16S rRNA gene sequence was observed to be 97.4% similar to <i>Roseomonas pecuniae</i> N75<sup>T</sup>. Genome-based phylogeny demonstrated that <i>Roseomonas harenae</i> CPCC 101081<sup>T</sup> and '<i>R. chloroacetimidivorans</i>' BUT-13<sup>T</sup>species are close relatives to strains SXEYE001<sup>T</sup> and SXEYE002. The average nucleotide identity between the strains and species of the genus <i>Roseomonas</i> was <95.0%. The G+C contents of strains SXEYE001<sup>T</sup> and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. 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Identification of Roseomonas xinghualingensis sp. nov. isolated from hospital air.
The genus Roseomonas comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of Roseomonas remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001T and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to 'Roseomonas chloroacetimidivorans' BUT 13T. The 16S rRNA gene sequence was observed to be 97.4% similar to Roseomonas pecuniae N75T. Genome-based phylogeny demonstrated that Roseomonas harenae CPCC 101081T and 'R. chloroacetimidivorans' BUT-13Tspecies are close relatives to strains SXEYE001T and SXEYE002. The average nucleotide identity between the strains and species of the genus Roseomonas was <95.0%. The G+C contents of strains SXEYE001T and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001T and SXEYE002 were indicative of a novel species within the genus Roseomonas, for which the name Roseomonas xinghualingensis sp. nov. is proposed, with SXEYE001T (=GDMCC 1.3626T=JCM 35902T) as the type strain.
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.