医院空气中玫瑰单胞菌的分离鉴定。

IF 2 3区 生物学 Q4 MICROBIOLOGY
Zhiming Kang, Wenjing Lei, Ji Pu, Lijun Zhao, Guowen Min, Yufeng Liu, Wenjuan Chang, Yuqian Gao, Kui Dong, Bin Sun
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引用次数: 0

摘要

玫瑰单胞菌属由广泛分布于环境中的革兰氏阴性菌组成,可引起人类感染。然而,对玫瑰单胞菌的基因组特征和致病性的研究仍然很少。从医院空气中分离到革兰氏阴性、中热、好氧和球虫两株新菌株(SXEYE001T和SXEYE002)。菌落在R2A板上28°C培养3天,菌落为圆形,边缘整齐,凸起,颜色为淡粉色。16S rRNA基因测序结果显示,这两株菌株与‘Roseomonas chloroacetimidivorans’BUT 13T有98.4%的相似性。16S rRNA基因序列与pecuniae玫瑰单胞菌N75T相似度为97.4%。基于基因组的系统发育表明,harenae Roseomonas CPCC 101081T和R. chloroacetimidivorans but - 13t与seye001t和seye002亲缘关系较近。玫瑰单胞菌属菌株和种间的平均核苷酸同源性分别为T和sye002,分别为67.8mol%和67.9mol%。主要极性脂质为二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱,辅酶Q-10为主要呼吸醌。综合基因型、表型、系统发育和系统基因组分析,我们认为菌株sye001t和sye002是玫瑰单胞菌属的新种,并以sye001t (=GDMCC 1.3626T=JCM 35902T)为型菌株,命名为xinghualingensis sp. nov。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of Roseomonas xinghualingensis sp. nov. isolated from hospital air.

The genus Roseomonas comprises Gram-negative bacteria widely distributed in the environment and can cause human infections. However, the genomic characteristics and pathogenicity of Roseomonas remain poorly studied. Two new Gram-negative, mesothermic, aerobic and coccoid strains (SXEYE001T and SXEYE002) were isolated from hospital air. Colonies grown on R2A plates at 28 °C for 3 days were round with neat, raised edges and a pale pink colour. Sequencing of the 16S rRNA gene revealed that the two strains are 98.4% similar to 'Roseomonas chloroacetimidivorans' BUT 13T. The 16S rRNA gene sequence was observed to be 97.4% similar to Roseomonas pecuniae N75T. Genome-based phylogeny demonstrated that Roseomonas harenae CPCC 101081T and 'R. chloroacetimidivorans' BUT-13Tspecies are close relatives to strains SXEYE001T and SXEYE002. The average nucleotide identity between the strains and species of the genus Roseomonas was <95.0%. The G+C contents of strains SXEYE001T and SXEYE002 were calculated to be 67.8mol% and 67.9mol%, respectively. The major polar lipids were determined to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, with coenzyme Q-10 serving as the primary respiratory quinone. Based on comprehensive genotypic, phenotypic, phylogenetic and phylogenomic analyses, we concluded that strains SXEYE001T and SXEYE002 were indicative of a novel species within the genus Roseomonas, for which the name Roseomonas xinghualingensis sp. nov. is proposed, with SXEYE001T (=GDMCC 1.3626T=JCM 35902T) as the type strain.

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来源期刊
CiteScore
5.20
自引率
21.40%
发文量
426
审稿时长
1 months
期刊介绍: Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names. The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including: Identification, characterisation and culture preservation Microbial evolution and biodiversity Molecular environmental work with strong taxonomic or evolutionary content Nomenclature Taxonomy and phylogenetics.
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