近端小管细胞在小鼠肾脏发育过程中参与Henle环的薄降肢。

IF 9.4 1区 医学 Q1 UROLOGY & NEPHROLOGY
Eunah Chung, Fariba Nosrati, Mike Adam, Andrew Potter, Mohammed Sayed, Christopher Ahn, Benjamin D Humphreys, Hee-Woong Lim, Yueh-Chiang Hu, S Steven Potter, Joo-Seop Park
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引用次数: 0

摘要

背景:Henle尿袢的细降肢对尿浓度至关重要,因为它有利于被动水重吸收。尽管它很重要,但人们对它在肾脏发育过程中是如何形成的知之甚少。方法:通过整合非突变发育小鼠肾脏的多个数据集,我们组装了一个大型单细胞RNA测序(scRNA-seq)数据集,以鉴定发育中的薄降肢细胞。为了测试这些细胞是否来自近端小管细胞,我们生成了近端小管特异性Cre系Slc34a1eGFPCre,并进行了谱系追踪。此外,考虑到转录因子Hnf4a直接与Aqp1基因结合,我们检测了Hnf4a的缺失是否会影响Aqp1在薄降肢细胞中的表达。结果:从我们的scRNA-seq数据集中,我们发现了一小群不同于近端小管和Henle环的厚升肢的细胞。这些细胞高表达瘦降肢标记基因,包括Aqp1和Bst1。值得注意的是,一部分近端小管细胞也表达了薄降肢标记基因,这表明近端小管细胞可能产生薄降肢细胞。使用Slc34a1eGFPCre系进行谱系追踪,我们发现,至少有一部分薄降肢细胞是近端小管细胞的后代。此外,成熟近端小管细胞形成所必需的转录因子Hnf4a的缺失,破坏了薄降肢细胞中Aqp1的正常表达,为近端小管细胞和薄降肢细胞之间的发育联系提供了额外的证据。结论:我们的研究结果揭示了薄降肢细胞的发育起源,并强调了Hnf4a在调节其形成中的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Proximal Tubule Cells Contribute to the Thin Descending Limb of the Loop of Henle during Mouse Kidney Development.
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来源期刊
Journal of The American Society of Nephrology
Journal of The American Society of Nephrology 医学-泌尿学与肾脏学
CiteScore
22.40
自引率
2.90%
发文量
492
审稿时长
3-8 weeks
期刊介绍: The Journal of the American Society of Nephrology (JASN) stands as the preeminent kidney journal globally, offering an exceptional synthesis of cutting-edge basic research, clinical epidemiology, meta-analysis, and relevant editorial content. Representing a comprehensive resource, JASN encompasses clinical research, editorials distilling key findings, perspectives, and timely reviews. Editorials are skillfully crafted to elucidate the essential insights of the parent article, while JASN actively encourages the submission of Letters to the Editor discussing recently published articles. The reviews featured in JASN are consistently erudite and comprehensive, providing thorough coverage of respective fields. Since its inception in July 1990, JASN has been a monthly publication. JASN publishes original research reports and editorial content across a spectrum of basic and clinical science relevant to the broad discipline of nephrology. Topics covered include renal cell biology, developmental biology of the kidney, genetics of kidney disease, cell and transport physiology, hemodynamics and vascular regulation, mechanisms of blood pressure regulation, renal immunology, kidney pathology, pathophysiology of kidney diseases, nephrolithiasis, clinical nephrology (including dialysis and transplantation), and hypertension. Furthermore, articles addressing healthcare policy and care delivery issues relevant to nephrology are warmly welcomed.
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