Amir Banari, Amit K Samanta, Anna Munke, Tim Laugks, Saša Bajt, Kay Grünewald, Thomas C Marlovits, Jochen Küpper, Filipe R N C Maia, Henry N Chapman, Dominik Oberthür, Carolin Seuring
{"title":"推进时间分辨结构生物学：低温电镜和x射线晶体学的最新策略。","authors":"Amir Banari, Amit K Samanta, Anna Munke, Tim Laugks, Saša Bajt, Kay Grünewald, Thomas C Marlovits, Jochen Küpper, Filipe R N C Maia, Henry N Chapman, Dominik Oberthür, Carolin Seuring","doi":"10.1038/s41592-025-02659-6","DOIUrl":null,"url":null,"abstract":"<p><p>Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could mutually benefit each other to advance our understanding of biology at the molecular and atomic scales.</p>","PeriodicalId":18981,"journal":{"name":"Nature Methods","volume":" ","pages":"1420-1435"},"PeriodicalIF":36.1000,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.\",\"authors\":\"Amir Banari, Amit K Samanta, Anna Munke, Tim Laugks, Saša Bajt, Kay Grünewald, Thomas C Marlovits, Jochen Küpper, Filipe R N C Maia, Henry N Chapman, Dominik Oberthür, Carolin Seuring\",\"doi\":\"10.1038/s41592-025-02659-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could mutually benefit each other to advance our understanding of biology at the molecular and atomic scales.</p>\",\"PeriodicalId\":18981,\"journal\":{\"name\":\"Nature Methods\",\"volume\":\" \",\"pages\":\"1420-1435\"},\"PeriodicalIF\":36.1000,\"publicationDate\":\"2025-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature Methods\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1038/s41592-025-02659-6\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature Methods","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41592-025-02659-6","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/1 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Advancing time-resolved structural biology: latest strategies in cryo-EM and X-ray crystallography.
Structural biology offers a window into the functionality of molecular machines in health and disease. A fundamental challenge lies in capturing both the high-resolution structural details and dynamic changes that are essential for function. The high-resolution methods of X-ray crystallography and electron cryo-microscopy (cryo-EM) are mainly used to study ensembles of static conformations but can also capture crucial dynamic transition states. Here, we review the latest strategies and advancements in time-resolved structural biology with a focus on capturing dynamic changes. We describe recent technology developments for time-resolved sample preparation and delivery in the cryo-EM and X-ray fields and explore how these technologies could mutually benefit each other to advance our understanding of biology at the molecular and atomic scales.
期刊介绍：
Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.
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