miR-369-3p通过靶向PTPN12调控肺癌细胞的耐药。

IF 1.9 4区医学 Q3 PHARMACOLOGY & PHARMACY

Pharmacogenomics Pub Date : 2025-05-14 DOI:10.1080/14622416.2025.2504864

Yan Wang, XiaoLi Wang, Jun Xu, Didi Zhang, YongFeng Cao

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引用次数: 0

摘要

目的：探讨miR-369-3p低表达对PC-9细胞对奥希替尼耐药的影响。方法：用奥西替尼建立PC-9/AZD9291细胞系。Real-time定量PCR检测PC-9和PC-9/AZD9291细胞中miR-369-3p的表达水平，Western blotting检测PTPN12蛋白的表达。通过双荧光素酶报告基因检测来研究miR-369-3p与PTPN12之间的靶标关系。CCK8检测评估miR-369-3p抑制对耐药的影响。结果：与PC-9细胞相比，PC-9/AZD9291细胞中miR-369-3p显著上调，PTPN12蛋白下调（p p p p）。结论：miR-369-3p通过调控PTPN12在PC-9/AZD9291细胞对奥西替尼的耐药中起关键作用。

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miR-369-3p regulates the drug resistance of lung cancer cells by targeting PTPN12.

Objective: To explore the impact of low miR-369-3p expression on the resistance of PC-9 cells to osimertinib.

Methods: The PC-9/AZD9291 cell line was established with osimertinib. Real-time quantitative PCR was employed to measure the expression levels of miR-369-3p in both PC-9 and PC-9/AZD9291 cells, and Western blotting was utilized to detect PTPN12 protein expression. A dual-luciferase reporter assay was conducted to investigate the target relationship between miR-369-3p and PTPN12. CCK8 assays were performed to evaluate the impact of miR-369-3p inhibition on drug resistance.

Results: In comparison to PC-9 cells, there was a significant upregulation of miR-369-3p and downregulation of PTPN12 protein in PC-9/AZD9291 cells (p < 0.05). Transfection with the miR-369-3p inhibitor resulted in decreased levels of miR-369-3p and increased expression of PTPN12 protein in PC-9/AZD9291 cells (p < 0.05). Conversely, transfection with miR-369 mimics led to an increase in miR-369-3p levels accompanied by a decrease in PTPN12 protein (p < 0.05). Notably, treatment with the miR-369-3p inhibitor lowered the IC50 value for PC-9/AZD9291 cells; however, following downregulation of PTPN12 using PTPN12-siRNA, sensitivity due to low expression of miR-369-3p was significantly diminished (p < 0.05).

Conclusion: miR-369-3p plays a crucial role in modulating drug resistance in PC-9/AZD9291 cells against osimertinib through regulation of PTPN12.

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来源期刊

Pharmacogenomics 医学-药学

CiteScore

3.40

自引率

9.50%

发文量

审稿时长

4-8 weeks

期刊介绍： Pharmacogenomics (ISSN 1462-2416) is a peer-reviewed journal presenting reviews and reports by the researchers and decision-makers closely involved in this rapidly developing area. Key objectives are to provide the community with an essential resource for keeping abreast of the latest developments in all areas of this exciting field. Pharmacogenomics is the leading source of commentary and analysis, bringing you the highest quality expert analyses from corporate and academic opinion leaders in the field.