植物质粒多样性的研究。perforans人口。

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
Amandeep Kaur, Albert Sunny, Jeffrey B Jones, Erica M Goss
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引用次数: 0

摘要

质粒是水平基因转移的关键驱动因素。这些遗传元素通过在细菌物种内部和物种之间转移有益的性状,促进细菌种群的多样化和对不断变化的环境的快速适应。黄单胞菌病。穿孔菌是一种毁灭性的植物病原体,引起番茄和辣椒的细菌性斑疹病。佛罗里达的病原体种群包含几个不同的遗传谱系,其相对频率不同。本研究的目的是表征质粒多样性和基因含量,以及质粒分布与染色体系统发育的关系。我们基于硅的质粒预测显示存在多种质粒,范围从~16 kb到~235 kb。基于共享k-mer含量的网络方法发现了十个具有高遗传相似性的不同质粒群(派系)。有趣的是,这些质粒团被限制在特定的系统发育簇中,这表明潜在的不相容或限制了质粒在簇之间的运动。一些预测的质粒携带编码III型分泌效应物(T3SEs)的毒力基因,包括转录激活物样效应物(TALEs)和与杀菌剂抗性相关的基因,如铜。我们还发现了质粒之间的基因重组,可能是由质粒内的转座子进行的。总的来说,这些结果为黄单胞菌的质粒多样性提供了基础的见解。穿孔与这些移动遗传元件在基因组动力学和病原体适应中的作用的含义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigating Plasmid Diversity in Xanthomonas euvesicatoria pv. perforans Population.

Plasmids are key drivers of horizontal gene transfer. These genetic elements promote diversification and rapid adaptation of bacterial populations to changing environments by transferring beneficial traits within and between bacterial species. Xanthomonas euvesicatoria pv. perforans is a devastating plant pathogen that causes bacterial spot disease in tomato and pepper. The pathogen population in Florida contains several distinct genetic lineages that differ in relative frequency. The objective of this study was to characterize plasmid diversity and gene content and plasmid distribution in relation to chromosomal phylogeny. Our in silico-based plasmid prediction revealed the presence of diverse plasmids ranging from approximately 16 to 235 kb. A network approach based on shared k-mer content uncovered 10 distinct plasmid groups with high genetic similarity (cliques). Interestingly, these plasmid cliques were confined to specific phylogenetic clusters, suggesting potential incompatibility or restricted plasmid movement between clusters. Some of the predicted plasmids carry virulence genes coding for type III secretion effectors, including transcription activator-like effectors and genes related to biocide resistance, such as copper. We also identified gene reshuffling between the plasmids, likely carried out by transposons present within them. Overall, these results provide foundational insights into plasmid diversity in X. euvesicatoria pv. perforans with implications for the role of these mobile genetic elements in genome dynamics and pathogen adaptation.

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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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