克罗恩病相关大肠杆菌BasRS参与菌毛表达，促进上皮细胞侵袭

IF 1.9 4区医学 Q4 IMMUNOLOGY

Microbiology and Immunology Pub Date : 2025-04-13 DOI:10.1111/1348-0421.13221

Tsuyoshi Miki, Masahiro Ito, Takeshi Haneda, Yun-Gi Kim

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引用次数: 0

摘要

克罗恩病相关的粘附-侵袭性大肠杆菌（AIEC）通过1型菌毛在肠道内定植。我们证明了双组分信号转导系统BasRS参与了AIEC菌株LF82中纤毛的表达，在缺乏BasRS的LF82突变体中，fimA的转录活性降低。因此，basRS突变体对HeLa细胞的侵袭性降低，通过引入以不依赖basRS的方式表达菌毛的质粒使其恢复。这些发现可能为开发aiec相关克罗恩病的治疗干预措施提供新的前景。

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Crohn's Disease‒Associated Escherichia coli BasRS Is Involved in Fimbriae Expression, Contributing to Epithelial Cell Invasion.

Crohn's disease‒associated adherent-invasive Escherichia coli (AIEC) colonizes the gut lumen through Type 1 fimbriae. We demonstrated that the two-component signal transduction system BasRS is involved in the expression of fimbriae in the AIEC strain LF82, as evidenced by the reduced transcriptional activity of fimA in an LF82 mutant lacking BasRS. Consequently, the basRS mutant showed decreased invasiveness to HeLa cells, which was restored by introducing a plasmid expressing fimbriae in a BasRS-independent manner. These findings may provide new prospects for developing therapeutic interventions for AIEC-related Crohn's disease.

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来源期刊

Microbiology and Immunology 医学-免疫学

CiteScore

5.20

自引率

3.80%

发文量

审稿时长

1 months

期刊介绍： Microbiology and Immunology is published in association with Japanese Society for Bacteriology, Japanese Society for Virology, and Japanese Society for Host Defense Research. It is peer-reviewed publication that provides insight into the study of microbes and the host immune, biological and physiological responses. Fields covered by Microbiology and Immunology include:Bacteriology|Virology|Immunology|pathogenic infections in human, animals and plants|pathogenicity and virulence factors such as microbial toxins and cell-surface components|factors involved in host defense, inflammation, development of vaccines|antimicrobial agents and drug resistance of microbes|genomics and proteomics.